Selenium speciation in paired serum and cerebrospinal fluid samples

Se speciation was performed in 24 individual paired serum and cerebrospinal fluid (CSF) samples from neurologically healthy persons. Strong anion exchange (SAX) separation, coupled to inductively coupled plasma–dynamic reaction cell–mass spectrometry (ICP-DRC-MS), was employed. Species identificatio...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2013-02, Vol.405 (6), p.1875-1884
Main Authors: Solovyev, Nikolay, Berthele, Achim, Michalke, Bernhard
Format: Article
Language:English
Subjects:
Analytical Chemistry
Anion exchange
Biochemistry
Cerebrospinal fluid
Characterization and Evaluation of Materials
Chemical properties
Chemical species
Chemistry
Chemistry and Materials Science
Composition
Correlation
Deviation
Enzymes
Food Science
Glutathione Peroxidase - blood
Glutathione Peroxidase - cerebrospinal fluid
Humans
Laboratory Medicine
Limit of Detection
Mass spectrometry
Mathematical analysis
Metabolism
Monitoring/Environmental Analysis
Nervous system
Organoselenium Compounds - blood
Organoselenium Compounds - cerebrospinal fluid
Original Paper
Oxidative stress
Parkinson's disease
Quality Control
Reference Values
Retention time
Selenium
Selenomethionine - blood
Selenomethionine - cerebrospinal fluid
Selenoprotein P - blood
Selenoprotein P - cerebrospinal fluid
Serum
Serum albumin
Serum Albumin - analysis
Serums
Speciation
Thioredoxin-Disulfide Reductase - blood
Thioredoxin-Disulfide Reductase - cerebrospinal fluid
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Summary:Se speciation was performed in 24 individual paired serum and cerebrospinal fluid (CSF) samples from neurologically healthy persons. Strong anion exchange (SAX) separation, coupled to inductively coupled plasma–dynamic reaction cell–mass spectrometry (ICP-DRC-MS), was employed. Species identification was done by standard matched retention time, standard addition and by size exclusion chromatography followed from SAX (2-D SEC-SAX-ICP-DRC-MS) and by SAX followed from CE-ICP-DRC-MS (2-D SAX-CE-ICP-DRC-MS). Limit of detection (LoD, 3 × standard deviation (SD) of noise) was in the range of 0.026–0.031 μg/L for all investigated species and thus was set uniformly to 0.032 μg/L. Quality control for total Se determination was performed by analysing control materials “human serum” and “urine”, where determined values met target values. Several Se species were found in both sample types having following median values (sequence: serum/CSF, each in μg Se/L): total Se, 58.39/0.86; selenoprotein P (SePP), 5.19/0.47; Se-methionine (SeM), 0.23/
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-012-6294-y