Elastin-like recombinamer-covered stents: Towards a fully biocompatible and non-thrombogenic device for cardiovascular diseases

[Display omitted] We explored the use of recently developed gels obtained by the catalyst free click reaction of elastin-like recombinamers (ELRs) to fabricate a new class of covered stents. The approach consists in embedding bare metal stents in the ELR gels by injection molding, followed by endoth...

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Bibliographic Details
Published in:Acta biomaterialia 2015, Vol.12, p.146-155
Main Authors: de Torre, Israel González, Wolf, Frederic, Santos, Mercedes, Rongen, Lisanne, Alonso, Matilde, Jockenhoevel, Stefan, Rodríguez-Cabello, José C., Mela, Petra
Format: Article
Language:English
Subjects:
Biocompatibility
Blood
Cardiovascular Diseases - therapy
Coated Materials, Biocompatible
Covering
Devices
Diseases
Elastin
Elastin-like recombinamers
Endothelialization
Gel
Gels
Human Umbilical Vein Endothelial Cells
Humans
Microscopy, Electron, Scanning
Restenosis
Stent
Stents
Surgical implants
Thrombosis
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Summary:[Display omitted] We explored the use of recently developed gels obtained by the catalyst free click reaction of elastin-like recombinamers (ELRs) to fabricate a new class of covered stents. The approach consists in embedding bare metal stents in the ELR gels by injection molding, followed by endothelialization under dynamic pressure and flow conditions in a bioreactor. The mechanical properties of the gels could be easily tuned by choosing the adequate concentration of the ELR components and their biofunctionality could be tailored by inserting specific sequences (RGD and REDV). The ELR-covered stents exhibited mechanical stability under high flow conditions and could undergo crimping and deployment without damage. The presence of RGD in the ELR used to cover the stent supported full endothelialization in less than 2weeks in vitro. Minimal platelet adhesion and fibrin adsorption were detected after exposure to blood, as shown by immunostaining and scanning electron microscopy. These results prove the potential of this approach towards a new and more effective generation of covered stents which exclude the atherosclerotic plaque from the blood stream and have high biocompatibility, physiological hemocompatibility and reduced response of the immune system.
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2014.10.029