Poly(3,4-ethylenedioxythiophene) coated chitosan modified disposable electrodes for DNA and DNA-drug interaction sensing

The need for sensitive, selective, rapid and low-cost detection systems for DNA and DNA-drug interactions are in crucial demand for diagnostics and real-world applications. This work details the preparation of poly(3,4-ethylenedioxythiophene) (PEDOT) coated chitosan (CHIT) and the use of PEDOT coate...

Full description

Saved in:
Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2014-11, Vol.123, p.825-830
Main Authors: Kuralay, Filiz, Demirci, Serpil, Kiristi, Melek, Oksuz, Lutfi, Oksuz, Aysegul Uygun
Format: Article
Language:English
Subjects:
Bridged Bicyclo Compounds, Heterocyclic - chemistry
Chitosan
Chitosan - chemistry
Coated electrodes
Deoxyribonucleic acid
Detection
DNA - chemistry
Drug Interactions
Drugs
Electrodes
Microscopy, Electron, Scanning
Polymers - chemistry
Radio frequencies
Voltammetry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The need for sensitive, selective, rapid and low-cost detection systems for DNA and DNA-drug interactions are in crucial demand for diagnostics and real-world applications. This work details the preparation of poly(3,4-ethylenedioxythiophene) (PEDOT) coated chitosan (CHIT) and the use of PEDOT coated CHIT modified disposable pencil graphite electrodes (PGEs) for DNA and DNA-anticancer drug interaction sensing. PEDOT coated CHIT (PEDOT/CHIT) was prepared with rotating plasma polymerization using radio frequency (RF: 13.56 MHz) power generator. Then, modification of PEDOT/CHIT onto PGE was performed. The use of the prepared electrode was carried out using differential pulse voltammetry (DPV). Cyclic voltammetry (CV) and scanning electron microscopy (SEM) were used to characterize the PEDOT/CHIT/PGE. The performance of the electrode was compared with CHIT/PGE and unmodified PGE. The electrode exhibited high sensitivity for the investigation of DNA sensing and DNA-anticancer drug interaction. Such disposable sensing platform hold considerable promise for diverse bioapplications.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2014.10.021