Cerium oxide nanoparticles protect primary mouse bone marrow stromal cells from apoptosis induced by oxidative stress

• Published in: Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology 2014-11, Vol.16 (11), p.1-12, Article 2697
• Main Authors: Zhang, Qun, Ge, Kun, Duan, Jianlei, Chen, Shizhu, Zhang, Ran, Zhang, Cuimiao, Wang, Shuxiang, Zhang, Jinchao
• Format: Article
• Language: English
• Subjects: Apoptosis; Bone marrow; Bones; Cellular; Cerium; Cerium oxide; Characterization and Evaluation of Materials; Chemistry and Materials Science; Cross-disciplinary physics: materials science; rheology; Exact sciences and technology; Hydrogen peroxide; Inorganic Chemistry; Intravenous administration; Lasers; Materials Science; Mice; Nanocrystalline materials; Nanoparticles; Nanoscale materials and structures: fabrication and characterization; Nanostructure; Nanotechnology; Nuclei; Optical Devices; Optics; Osteoporosis; Oxidative stress; Photonics; Physical Chemistry; Physics; Research Paper; Synergistic effect
• ISSN: 1388-0764; 1572-896X
• DOI: 10.1007/s11051-014-2697-3

Cerium oxide nanoparticles (nanoceria) have been widely used in industries and biomedical fields due to its unique properties. Previous biodistribution studies of nanoceria in vivo have shown that they are accumulated in the bone of mice after intravenous administration, about 20 % of the total intake, however, the potential effect and the mechanism of nanoceria on bone metabolism are not well-understood. Our results showed that both 25 and 50 nm nanceria decreased the damage of cell viability induced by H 2 O 2 in a dose-dependent manner. The apoptosis ratio of pre-incubated group with nanoceria was lower than the H 2 O 2 group. The cellular uptake studies indicated that there was a dose-dependent accumulation of both two size nanoparticles in bone marrow stromal cells. Nanoceria could be uptaken by cells due to the synergistic effect of multiple endocytosis mechanisms, and then evenly distributed in the cytoplasm without entering the nucleus. Our results suggest that nanoceria could reduce intracellular ROS level induced by H 2 O 2 in a dose-dependent manner, moreover, maintain the normal function of mitochondria, suggesting nanoceria may have potent applications for preventing or treating osteoporosis.