Tetracycline-inducible gene expression system in Leishmania mexicana

•We established Tet-regulated gene expression system in Leishmania mexicana.•Gene expression in our system is time- and dose-dependent.•Protein expression background is low. Here we present a T7-driven, tetracycline-inducible system for protein expression in human pathogen Leishmania mexicana. The g...

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Bibliographic Details
Published in:Molecular and biochemical parasitology 2014-11, Vol.198 (1), p.11-13
Main Authors: Kraeva, Natalya, Ishemgulova, Aygul, Lukeš, Julius, Yurchenko, Vyacheslav
Format: Article
Language:English
Subjects:
Gene Expression
Humans
Leishmania mexicana
Leishmania mexicana - drug effects
Leishmania mexicana - genetics
Leishmania mexicana - metabolism
Leishmaniasis, Cutaneous - parasitology
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Tet-inducible system
Tetracycline - pharmacology
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Summary:•We established Tet-regulated gene expression system in Leishmania mexicana.•Gene expression in our system is time- and dose-dependent.•Protein expression background is low. Here we present a T7-driven, tetracycline-inducible system for protein expression in human pathogen Leishmania mexicana. The gene expression in this strain is tightly regulated and dose- and time-dependent. This system can be widely used by the parasitology community to analyze effects of genes of interest on biology, physiology and virulence of parasites causing cutaneous leishmaniases.
ISSN:0166-6851
1872-9428
DOI:10.1016/j.molbiopara.2014.11.002