Regulation and Production of Interleukin 35 Subunits, p35 and EBI3, in Human Bronchial Epithelial Cells

Methods Primary normal human bronchial epithelial (NHBE) cells were stimulated with cytokines and TLR ligands and mRNA and protein expression of p35 and EBI3 were determined by real-time RT-PCR and Western blot.

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Bibliographic Details
Published in:Journal of allergy and clinical immunology 2015-02, Vol.135 (2), p.AB163-AB163
Main Authors: Kato, Atsushi, PhD, Takabayashi, Tetsuji, MD, Klingler, Aiko I., PhD, Poposki, Julie A., MS
Format: Article
Language:English
Subjects:
Allergy and Immunology
Ligands
Medical research
Online Access:Get full text
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Description
Summary:Methods Primary normal human bronchial epithelial (NHBE) cells were stimulated with cytokines and TLR ligands and mRNA and protein expression of p35 and EBI3 were determined by real-time RT-PCR and Western blot.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2014.12.1472