Prototype of oligonucleotide microarray for detection of pathogens relating to arena- and filoviridae families

A prototype of an oligonucleotide microarray for detection of the Lassa, Junin, Machupo, Guanarito viruses (Arenaviridae family), Ebola and Marburg viruses (Filoviridae family) has been presented. The design of primers and probes for hybridization was performed using an original approach based on th...

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Bibliographic Details
Published in:Russian journal of bioorganic chemistry 2015, Vol.41 (1), p.46-56
Main Authors: Zhirnov, I. V., Ryabinin, V. A., Sinyakov, A. N., Ternovoy, V. A., Shikov, A. N.
Format: Article
Language:English
Subjects:
Arenaviridae
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Ebola virus
Filoviridae
Life Sciences
Marburg virus
Organic Chemistry
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Summary:A prototype of an oligonucleotide microarray for detection of the Lassa, Junin, Machupo, Guanarito viruses (Arenaviridae family), Ebola and Marburg viruses (Filoviridae family) has been presented. The design of primers and probes for hybridization was performed using an original approach based on the analysis of amino acid sequences of virus proteins (nucleocapsid protein for the Junin, Guanarito, and Machupo viruses and RNA-dependent RNA-polymerase for the Lassa, Ebola, and Marburg viruses) with the subsequent decoding of the identified unique peptides in the corresponding sets of oligonucleotides.
ISSN:1068-1620
1608-330X
DOI:10.1134/S1068162014050136