Genetic diversity analysis of Perinereis aibuhitensis based on ISSR and SRAP markers of Chinese coast populations

• Published in: Biochemical systematics and ecology 2014-12, Vol.57, p.262-269
• Main Authors: Liu, Fei, Guo, Qiao-sheng, Shi, Hong-zhuan, Lv, Fu, Yu, Ye-bing, Lv, Lin-lan, Huang, Jin-tian, Wang, Ai-ming, Liang, Hui-xing
• Format: Article
• Language: English
• Subjects: Genetic diversity; ISSR; Molecular markers; Perinereis aibuhitensis; SRAP
• ISSN: 0305-1978; 1873-2925
• DOI: 10.1016/j.bse.2014.08.025

The objective of this study was to obtain an overview of the genetic relationships within Perinereis aibuhitensis using Inter-Simple Sequence Repeat (ISSR) and Sequence-Related Amplified Polymorphism (SRAP) markers that were derived from related populations residing in the Chinese coasts. The percentage of polymorphic bands, Nei's gene diversity and Shannon's information index revealed a high level of genetic diversity at the species level. The analysis of molecular variance revealed that 81.22% (ISSR) and 76.29% (SRAP) of variability were partitioned among individuals within populations, which indicated the coherent trend by Nei's genetic differentiation (Gst) (0.2568/0.2876). The gene flow number (Nm) was 1.4470/1.2385, which indicated that there was limited gene exchange between populations. The phylogenetic tree of the ten P. aibuhitensis populations was separated into four major clusters using the neighbor-joining (NJ) method. These results provide a simple and useful basis for P. aibuhitensis germplasm research and aquaculture breeding. •We assayed the population genetics of Perinereis aibuhitensis using ISSR and SRAP markers.•The genetic population structures of P. aibuhitensis with a high level of genetic diversity at the species level.•These results provide a simple and useful basis for P. aibuhitensis germplasm research and aquaculture breeding.