Effects of TGF‐ β 1 on OPG/RANKL Expression of Cementoblasts and Osteoblasts Are Similar without Stress but Different with Mechanical Compressive Stress

• Published in: TheScientificWorld 2015-01, Vol.2015 (1)
• Main Authors: Yang, Xianrui, Wang, Yanmin, Han, Xianglong, Shu, Rui, Chen, Tian, Zeng, Huan, Xu, Xin, Huang, Lan, Ren, Aishu, Song, Jinlin, Cao, Li, Bai, Ding
• Format: Article
• Language: English
• ISSN: 2356-6140; 1537-744X
• DOI: 10.1155/2015/718180

Introduction. This study aimed to explore the effects of TGF‐ β 1 on regulating activities of cementoblasts and osteoblasts with or without stress. Material and Methods . Human recombinant TGF‐ β 1 was added with different doses. Immunohistochemical test of osteoprotegerin (OPG)/receptor activator of nuclear factor‐kappaB ligand (RANKL) and Alizarin Red‐S staining were conducted. Mechanical compressive stress was obtained by increasing the pressure of gaseous phase. OPG/RANKL expression was detected in both cells through quantitative real‐time PCR. Results . Similar significant differences ( P < 0.05) existed in OPG/RANKL change with increasing concentration of TGF‐ β 1 without mechanical stress for cementoblasts and osteoblasts. However, under 3 h stress, OPG increased and RANKL decreased significantly ( P < 0.01) but with similar OPG/RANKL change. Moreover, under 24 h stress, OPG change exhibited no difference ( P > 0.05), but RANKL decreased significantly ( P < 0.01) at 10 and 100 ng/mL TGF‐ β 1 in cementoblasts. In osteoblasts, OPG increased significantly ( P < 0.01) at 10 and 100 ng/mL, whereas RANKL decreased with statistical difference ( P < 0.05) at 1 and 10 ng/mL. Conclusions . The effects of TGF‐ β 1 on OPG/RANKL expression of cementoblasts and osteoblasts are similar even without mechanical stress. However, these effects are different under mechanical compressive stress.