Increased Expression of Leukotriene C4 Synthase Is a Feature of Circulating CD34+ Hematopoietic Stem Cells but Not Circulating Eosinophils in Aspirin-Exacerbated Respiratory Disease (AERD)

Methods Eosinophils and CD34+ cells were enriched from peripheral blood leukocytes obtained from control, aspirin-tolerant asthmatic, and AERD subjects. mRNA was extracted and subjected to reverse transcription followed by quantitative polymerase chain reaction (qPCR) to evaluate expression of genes...

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Bibliographic Details
Published in:Journal of allergy and clinical immunology 2015-02, Vol.135 (2), p.AB222-AB222
Main Authors: Baker, Mary Grace, MD, Negri, Julie, BS, Steinke, John W., PhD FAAAAI, Borish, Larry, MD, FAAAAI
Format: Article
Language:English
Subjects:
Allergy and Immunology
Aspirin
Bone marrow
Enzymes
Metabolism
Metabolites
Stem cells
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Summary:Methods Eosinophils and CD34+ cells were enriched from peripheral blood leukocytes obtained from control, aspirin-tolerant asthmatic, and AERD subjects. mRNA was extracted and subjected to reverse transcription followed by quantitative polymerase chain reaction (qPCR) to evaluate expression of genes related to AA metabolism and responsiveness.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2014.12.1662