Molecular detection of Leishmania isolated from Cutaneous leishmaniasis patients in Jask County,Hormozgan Province,Southern Iran,2008

• Published in: Asian Pacific journal of tropical medicine 2012-07, Vol.5 (7), p.514-517
• Main Authors: Azizi, Koroush, Soltani, Aboozar, Alipour, Hamzeh
• Format: Article
• Language: English
• Subjects: Cross-Sectional Studies; Cutaneous; DNA, Protozoan - genetics; Humans; Iran; Iran - epidemiology; Jask; Leishmania; Leishmania major; Leishmania major - genetics; Leishmania major - isolation & purification; Leishmaniasis; Leishmaniasis, Cutaneous - diagnosis; Leishmaniasis, Cutaneous - epidemiology; Leishmaniasis, Cutaneous - genetics; major; Molecular Diagnostic Techniques - methods; Nested; Nested PCR; PCR; Polymerase Chain Reaction - methods; Zoonotic; Zoonotic Cutaneous Leishmaniasis
• ISSN: 1995-7645; 2352-4146
• DOI: 10.1016/S1995-7645(12)60090-X

<正>Objective:To investigate on patients leishmanial infections in Jask County.Methods: Impression smears were prepared from patients in 2008,all,were chequed for leishmanial infection by microscopy and molecular assays.Whole D.NA was extracted using Proteinase K and Phenol/Chloroform/Isoamyl alcohol method.The variable segment on minicircles of kineloplasl DIVA was amplified via a Nested-PCR technique using species-specific primers(LIN R4-LIN 17 -Lin 19).Results:A total of 40 smears were prepared from 20 patients,from which,eight samples (40%) were positive for leishman body by microscopic method,while,18 samples(90%) were positive,molecularly.The parasite was identified as Leishmania major(L major).Conclusions: Zoonotic or Rural cutaneous leishmaniasis is endemic in Jask County whose pathogen is L major.Molecular assays using specific primers are very accurate and more sensitive and specific than microscopy which is time consuming and needs master mieroscopists.