Investigation of caspase-1 activity and interleukin-1β production in murine macrophage cell lines infected with Leishmania major

To investigate the caspase-1 dependent inflammatory pathway activity and interleukin-1β (IL-1β) secretion in murine macrophage cell lines J774G8 infected with Leishmania major (L. major) using caspase-1 activity assay and ELISA. Novy-MacNeal-Nicolle biphasic medium was applied to produce promastigot...

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Bibliographic Details
Published in:Asian Pacific journal of tropical medicine 2014-09, Vol.7, p.S70-S73
Main Authors: Sani, Mohammad Reza Mahmoudian, Moghaddam, Mehrdad Moosazadeh, Aghamollaei, Hossein, Hassanpour, Kazem, Taheri, Ramezan Ali, Farnoosh, Gholamreza
Format: Article
Language:English
Subjects:
Caspase-1 activity
Interleukin-1β
Leishmania major
Macrophage
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Summary:To investigate the caspase-1 dependent inflammatory pathway activity and interleukin-1β (IL-1β) secretion in murine macrophage cell lines J774G8 infected with Leishmania major (L. major) using caspase-1 activity assay and ELISA. Novy-MacNeal-Nicolle biphasic medium was applied to produce promastigote form of L. major. Metacyclic promastigotes in the stationary phase were applied to infect macrophage. Caspase-1 activity and IL-1β secretion were assessed by the CPP32/caspase-1 fluorometric protease assay and ELISA IL-1β kits, respectively, with time intervals of 6, 18 and 30 h. Our study showed an increase in caspase-1 activity and IL-1β secretion in infected samples compared to non-infected macrophages. The highest increase in IL-1β production was observed after 6 h of infection. These results arise that the activation of inflammasome pathway could be one of the innate immunity pathways against L. major.
ISSN:1995-7645
2352-4146
DOI:10.1016/S1995-7645(14)60205-4