Chryseobacterium polytrichastri sp. nov., isolated from a moss (Polytrichastrum formosum), and emended description of the genus Chryseobacterium

• Published in: Antonie van Leeuwenhoek 2015-02, Vol.107 (2), p.403-410
• Main Authors: Chen, Xin Yao, Zhao, Ran, Chen, Zhi Ling, Liu, Lei, Li, Xue Dong, Li, Yan Hong
• Format: Article
• Language: English
• Subjects: Bacteria; Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; Bryophyta - growth & development; Bryophyta - microbiology; Chryseobacterium; Chryseobacterium - classification; Chryseobacterium - genetics; Chryseobacterium - isolation & purification; Cluster Analysis; Cytosol - chemistry; Deoxyribonucleic acid; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; fatty acids; Fatty Acids - analysis; Glaciers; Ice Cover; Life Sciences; Lipids; Medical Microbiology; menaquinones; Microbiology; Molecular Sequence Data; Mosses; mosses and liverworts; new species; Nucleic Acid Hybridization; nucleotide sequences; Original Paper; Phospholipids - analysis; Phylogenetics; Phylogeny; Plant Sciences; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; sequence homology; Soil Science & Conservation; Taxonomy; Tibet; Vitamin K 2 - analysis
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-014-0338-6

A Gram-stain negative, rod-shaped and non-endospore forming bacterium, designated strain YG4-6ᵀ, was isolated from Polytrichastrum formosum collected from Gawalong glacier in Tibet, China and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of strain YG4-6ᵀwere identified as iso-C₁₅:₀(29.3 %), summed feature 3 (C₁₆:₁ω7c and/or C₁₆:₁ω6c as defined by MIDI, 23.5 %) and iso-C₁₇:₀3-OH (16.5 %). The major polar lipids were found to consist of five unidentified aminolipids and three unidentified lipids. Strain YG4-6ᵀwas found to contain MK-6 as the dominant menaquinone and the G+C content of its genomic DNA was determined to be 37.3 mol%. The phylogenetic analysis based on 16S rRNA gene sequences showed that strain YG4-6ᵀis affiliated to Chryseobacterium species, and its closest related species were Chryseobacterium aahli T68ᵀ(97.9 % sequence similarity), Chryseobacterium zeae JM-1085ᵀ(97.8 % sequence similarity), Chryseobacterium yeoncheonense DCY67ᵀ(97.6 % sequence similarity) and Chryseobacterium soldanellicola NBRC 100864ᵀ(97.2 % sequence similarity). However, the DNA–DNA relatedness values between these strains and strain YG4-6ᵀwere found to be clearly below 70 %. Based on the phylogenetic inference and phenotypic data, strain YG4-6ᵀis considered to represent a novel species of the genus Chryseobacterium, for which the name Chryseobacterium polytrichastri sp. nov. is proposed. The type strain is YG4-6ᵀ(=CGMCC 1.12491ᵀ = DSM 26899ᵀ). An emended description of the genus Chryseobacterium is also proposed.