Characteristics of very virulent infectious bursal disease viruses isolated from Chinese broiler chickens (2012–2013)

•VP2 genes of nine vvIBDVs were phylogenetically analyzed in this study.•Nine isolates were highly similar to each other and other reference vvIBDVs.•Our results confirm the circulation of vvIBDVs in Chinese broiler chicken farms.•Our results confirm the nucleotide substitution rate in VP2 is relati...

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Bibliographic Details
Published in:Acta tropica 2015-01, Vol.141 (Pt A), p.128-134
Main Authors: Xu, Mei-yu, Lin, Shu-yi, Zhao, Ye, Jin, Ji-hui, Tang, Na, Zhang, Guo-zhong
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Birnaviridae Infections - virology
Chickens
China
Evolution, Molecular
Field isolates
Infectious bursal disease virus
Infectious bursal disease virus - genetics
Phylogenetic analysis
Phylogeny
Poultry Diseases - virology
Reverse Transcriptase Polymerase Chain Reaction
Viral Structural Proteins - genetics
Viral Structural Proteins - metabolism
VP2
vvIBDV
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Summary:•VP2 genes of nine vvIBDVs were phylogenetically analyzed in this study.•Nine isolates were highly similar to each other and other reference vvIBDVs.•Our results confirm the circulation of vvIBDVs in Chinese broiler chicken farms.•Our results confirm the nucleotide substitution rate in VP2 is relatively slow. The objective of this study was to characterize the infectious bursal disease viruses (IBDVs) circulating in broiler chicken farms in China between 2012 and 2013. The VP2 gene sequences of nine newly isolated IBDVs, obtained using reverse transcriptase polymerase chain reaction, were determined and compared with worldwide reference isolates, which have been previously well characterized. Phylogenetic analysis revealed that the nine broiler IBDV isolates are closely related to very virulent IBDV (vvIBDV) strains. Analysis of the predicted amino acid sequences of VP2 from the nine vvIBDVs isolated from the broilers revealed that they share 99.2 to 100% sequence similarity. Additionally, amino acids A222, I242, I256, I294 and S299 of VP2 that are conserved among previously characterized vvIBDV strains are also encoded by the nine isolates. This study confirms the circulation of vvIBDVs in Chinese broiler chicken farms experienced slow evolution and was relatively stable in China.
ISSN:0001-706X
1873-6254
DOI:10.1016/j.actatropica.2014.10.003