Isolation, identification and functional profile of excretory–secretory peptides from Onchocerca ochengi

•Determination of peptides in female O. ochengi secretome.•Peptides analyzed for potential biological activity.•Three peptides identified with substantial antibacterial activity. Parasitic helminths excrete or secrete a variety of functional molecules into the internal milieu of their mammalian host...

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Bibliographic Details
Published in:Acta tropica 2015-02, Vol.142, p.156-166
Main Authors: Eberle, Raphael, Brattig, Norbert W., Trusch, Maria, Schlüter, Hartmut, Achukwi, Mbunkah Daniel, Eisenbarth, Albert, Renz, Alfons, Liebau, Eva, Perbandt, Markus, Betzel, Christian
Format: Article
Language:English
Subjects:
Animals
Antibacterial activity
Arthropoda
Cross Reactions
Excretory–secretory products
Female
Filaria
Functional peptides
Helminth Proteins - secretion
Onchocerca
Onchocerca - physiology
Onchocerca ochengi
Onchocerciasis - prevention & control
Onchocerciasis - transmission
Simuliidae - parasitology
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Summary:•Determination of peptides in female O. ochengi secretome.•Peptides analyzed for potential biological activity.•Three peptides identified with substantial antibacterial activity. Parasitic helminths excrete or secrete a variety of functional molecules into the internal milieu of their mammalian hosts and arthropod vectors which reveal distinct immunomodulatory and other biological activities. We identified and initially characterized the low molecular weight peptide composition of the secretome from the filarial parasite Onchocerca ochengi. A total of 85 peptides were purified by liquid chromatography and further characterized by mass spectrometry. 72 of these peptides were derived from already described Onchocerca proteins and 13 peptide sequences are included in the sequence of uncharacterized proteins. Three peptides, similar to host defense peptides, revealed antibacterial activity. The present analysis confirms the putative involvement of low molecular weight compounds in the parasite-host cross-talk.
ISSN:0001-706X
1873-6254
DOI:10.1016/j.actatropica.2014.11.015