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correlation of Tim-3 and IFN-γ expressions in mice infected with Toxoplasma gondii during gestation
The immunoinhibitory receptor T cell immunoglobulin domain and mucin domain-1 (Tim-1) and Tim-3 participate in the regulation of Th immune response as well as innate immunity. However, there is no report about the expression of Tim genes in Toxoplasma gondii-infected experimental models during pregn...
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| Published in: | Parasitology research (1987) 2015, Vol.114 (1), p.125-132 |
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| container_title | Parasitology research (1987) |
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| creator | Fu, Xiaoyin Wu, Bin Huang, Bo Zheng, Huanqin Huang, Shiguang Gan, Yan Shen, Jilong Lun, Zhao-Rong Lu, Fangli |
| description | The immunoinhibitory receptor T cell immunoglobulin domain and mucin domain-1 (Tim-1) and Tim-3 participate in the regulation of Th immune response as well as innate immunity. However, there is no report about the expression of Tim genes in Toxoplasma gondii-infected experimental models during pregnancy. In this study, Kunming outbred pregnant mice were infected with RH strain of T. gondii through vagina at days 10 to 16 of gestation, and the mRNA expressions of Tim-1, Tim-3, interleukin (IL)-4, and interferon (IFN)-γ in the placentas, uteri, and draining lumber aortic lymph nodes (LALNs) at day 18 of gestation were analyzed using quantitative real-time PCR (qRT-PCR). Compared with uninfected pregnant controls, significantly increased levels of IFN-γ and Tim-3 were detected in the placentas (P |
| doi_str_mv | 10.1007/s00436-014-4167-3 |
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However, there is no report about the expression of Tim genes in Toxoplasma gondii-infected experimental models during pregnancy. In this study, Kunming outbred pregnant mice were infected with RH strain of T. gondii through vagina at days 10 to 16 of gestation, and the mRNA expressions of Tim-1, Tim-3, interleukin (IL)-4, and interferon (IFN)-γ in the placentas, uteri, and draining lumber aortic lymph nodes (LALNs) at day 18 of gestation were analyzed using quantitative real-time PCR (qRT-PCR). Compared with uninfected pregnant controls, significantly increased levels of IFN-γ and Tim-3 were detected in the placentas (P < 0.001), uteri (P = 0.003 and P = 0.017, respectively), and LALNs (P = 0.003 and P = 0.025, respectively) of T. gondii-infected mice; there were positive and significant correlations between Tim-3 and IFN-γ mRNA expression levels in the placentas (R² = 0.6331, P = 0.0011), uteri (R² = 0.5658, P = 0.003), and LALNs (R² = 0.5583, P = 0.0033) of infected mice. Tim-1 (P = 0.002) and IL-4 (P = 0.003) expressions were significantly increased in the placentas, but Tim-1 were significantly decreased in the uteri (P = 0.013) and LALNs (P < 0.001) of infected pregnant mice in comparison of uninfected pregnant controls. Our data suggested that Tim-3 may play a regulatory role in T. gondii-infected pregnant mouse model.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-014-4167-3</identifier><identifier>PMID: 25270237</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>animal disease models ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Female ; gene expression ; Gene Expression Regulation - physiology ; genes ; Hepatitis A Virus Cellular Receptor 2 ; immune response ; immunoglobulins ; Immunology ; innate immunity ; interferon-gamma ; Interferon-gamma - genetics ; Interferon-gamma - metabolism ; interleukin-4 ; lumber ; lymph nodes ; Medical Microbiology ; messenger RNA ; Mice ; Microbiology ; mucins ; Original Paper ; Pregnancy ; Pregnancy Complications, Parasitic - immunology ; Pregnancy Complications, Parasitic - metabolism ; quantitative polymerase chain reaction ; Real-Time Polymerase Chain Reaction ; Receptors, Virus - genetics ; Receptors, Virus - metabolism ; reverse transcriptase polymerase chain reaction ; Toxoplasma ; Toxoplasma gondii ; Toxoplasmosis, Animal - metabolism ; vagina</subject><ispartof>Parasitology research (1987), 2015, Vol.114 (1), p.125-132</ispartof><rights>Springer-Verlag Berlin Heidelberg 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-34272b9192798477b13f7b4586ba144f497065e6ab0840e1ee1f1d6b1f16ffd33</citedby><cites>FETCH-LOGICAL-c401t-34272b9192798477b13f7b4586ba144f497065e6ab0840e1ee1f1d6b1f16ffd33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25270237$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fu, Xiaoyin</creatorcontrib><creatorcontrib>Wu, Bin</creatorcontrib><creatorcontrib>Huang, Bo</creatorcontrib><creatorcontrib>Zheng, Huanqin</creatorcontrib><creatorcontrib>Huang, Shiguang</creatorcontrib><creatorcontrib>Gan, Yan</creatorcontrib><creatorcontrib>Shen, Jilong</creatorcontrib><creatorcontrib>Lun, Zhao-Rong</creatorcontrib><creatorcontrib>Lu, Fangli</creatorcontrib><title>correlation of Tim-3 and IFN-γ expressions in mice infected with Toxoplasma gondii during gestation</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>The immunoinhibitory receptor T cell immunoglobulin domain and mucin domain-1 (Tim-1) and Tim-3 participate in the regulation of Th immune response as well as innate immunity. However, there is no report about the expression of Tim genes in Toxoplasma gondii-infected experimental models during pregnancy. In this study, Kunming outbred pregnant mice were infected with RH strain of T. gondii through vagina at days 10 to 16 of gestation, and the mRNA expressions of Tim-1, Tim-3, interleukin (IL)-4, and interferon (IFN)-γ in the placentas, uteri, and draining lumber aortic lymph nodes (LALNs) at day 18 of gestation were analyzed using quantitative real-time PCR (qRT-PCR). Compared with uninfected pregnant controls, significantly increased levels of IFN-γ and Tim-3 were detected in the placentas (P < 0.001), uteri (P = 0.003 and P = 0.017, respectively), and LALNs (P = 0.003 and P = 0.025, respectively) of T. gondii-infected mice; there were positive and significant correlations between Tim-3 and IFN-γ mRNA expression levels in the placentas (R² = 0.6331, P = 0.0011), uteri (R² = 0.5658, P = 0.003), and LALNs (R² = 0.5583, P = 0.0033) of infected mice. Tim-1 (P = 0.002) and IL-4 (P = 0.003) expressions were significantly increased in the placentas, but Tim-1 were significantly decreased in the uteri (P = 0.013) and LALNs (P < 0.001) of infected pregnant mice in comparison of uninfected pregnant controls. Our data suggested that Tim-3 may play a regulatory role in T. gondii-infected pregnant mouse model.</description><subject>animal disease models</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Female</subject><subject>gene expression</subject><subject>Gene Expression Regulation - physiology</subject><subject>genes</subject><subject>Hepatitis A Virus Cellular Receptor 2</subject><subject>immune response</subject><subject>immunoglobulins</subject><subject>Immunology</subject><subject>innate immunity</subject><subject>interferon-gamma</subject><subject>Interferon-gamma - genetics</subject><subject>Interferon-gamma - metabolism</subject><subject>interleukin-4</subject><subject>lumber</subject><subject>lymph nodes</subject><subject>Medical Microbiology</subject><subject>messenger RNA</subject><subject>Mice</subject><subject>Microbiology</subject><subject>mucins</subject><subject>Original Paper</subject><subject>Pregnancy</subject><subject>Pregnancy Complications, Parasitic - immunology</subject><subject>Pregnancy Complications, Parasitic - metabolism</subject><subject>quantitative polymerase chain reaction</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Receptors, Virus - genetics</subject><subject>Receptors, Virus - metabolism</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>Toxoplasma</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis, Animal - metabolism</subject><subject>vagina</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkcFu1TAQRS1ERR-FD2ADXrIxnbEdO1miikKlii54XVtOYj9cJXGwE1G-i__gm_AjhSXqxmPJZ-6M7yXkFcI7BNDnGUAKxQAlk6g0E0_IDqXgDJuqekp20JQ7IIpT8jznOwDUSspn5JRXXAMXekf6LqbkBruEONHo6T6MTFA79fTq8jP79ZO6-zm5nMtzpmGiY-hcqd51i-vp97B8pft4H-fB5tHSQ5z6EGi_pjAd6MHl5Y_wC3Li7ZDdy4d6Rm4vP-wvPrHrm49XF--vWScBFyYk17xtsOG6qaXWLQqvW1nVqrUopZeNBlU5ZVuoJTh0Dj32qi2n8r4X4oy83XTnFL-tZboZQ-7cMNjJxTUbVApEU_6uH4FKrKtaVE1BcUO7FHNOzps5hdGmHwbBHHMwWw6m5GCOOZjjJq8f5Nd2dP2_jr_GF4BvQJ6PXrlk7uKapuLOf1XfbE3eRmMPKWRz-4UDVgBQ17Ls-hsypJt_</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>Fu, Xiaoyin</creator><creator>Wu, Bin</creator><creator>Huang, Bo</creator><creator>Zheng, Huanqin</creator><creator>Huang, Shiguang</creator><creator>Gan, Yan</creator><creator>Shen, Jilong</creator><creator>Lun, Zhao-Rong</creator><creator>Lu, Fangli</creator><general>Springer-Verlag</general><general>Springer Berlin Heidelberg</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>M7N</scope></search><sort><creationdate>2015</creationdate><title>correlation of Tim-3 and IFN-γ expressions in mice infected with Toxoplasma gondii during gestation</title><author>Fu, Xiaoyin ; Wu, Bin ; Huang, Bo ; Zheng, Huanqin ; Huang, Shiguang ; Gan, Yan ; Shen, Jilong ; Lun, Zhao-Rong ; Lu, Fangli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-34272b9192798477b13f7b4586ba144f497065e6ab0840e1ee1f1d6b1f16ffd33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>animal disease models</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Female</topic><topic>gene expression</topic><topic>Gene Expression Regulation - physiology</topic><topic>genes</topic><topic>Hepatitis A Virus Cellular Receptor 2</topic><topic>immune response</topic><topic>immunoglobulins</topic><topic>Immunology</topic><topic>innate immunity</topic><topic>interferon-gamma</topic><topic>Interferon-gamma - genetics</topic><topic>Interferon-gamma - metabolism</topic><topic>interleukin-4</topic><topic>lumber</topic><topic>lymph nodes</topic><topic>Medical Microbiology</topic><topic>messenger RNA</topic><topic>Mice</topic><topic>Microbiology</topic><topic>mucins</topic><topic>Original Paper</topic><topic>Pregnancy</topic><topic>Pregnancy Complications, Parasitic - immunology</topic><topic>Pregnancy Complications, Parasitic - metabolism</topic><topic>quantitative polymerase chain reaction</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Receptors, Virus - genetics</topic><topic>Receptors, Virus - metabolism</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>Toxoplasma</topic><topic>Toxoplasma gondii</topic><topic>Toxoplasmosis, Animal - metabolism</topic><topic>vagina</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fu, Xiaoyin</creatorcontrib><creatorcontrib>Wu, Bin</creatorcontrib><creatorcontrib>Huang, Bo</creatorcontrib><creatorcontrib>Zheng, Huanqin</creatorcontrib><creatorcontrib>Huang, Shiguang</creatorcontrib><creatorcontrib>Gan, Yan</creatorcontrib><creatorcontrib>Shen, Jilong</creatorcontrib><creatorcontrib>Lun, Zhao-Rong</creatorcontrib><creatorcontrib>Lu, Fangli</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fu, Xiaoyin</au><au>Wu, Bin</au><au>Huang, Bo</au><au>Zheng, Huanqin</au><au>Huang, Shiguang</au><au>Gan, Yan</au><au>Shen, Jilong</au><au>Lun, Zhao-Rong</au><au>Lu, Fangli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>correlation of Tim-3 and IFN-γ expressions in mice infected with Toxoplasma gondii during gestation</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2015</date><risdate>2015</risdate><volume>114</volume><issue>1</issue><spage>125</spage><epage>132</epage><pages>125-132</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>The immunoinhibitory receptor T cell immunoglobulin domain and mucin domain-1 (Tim-1) and Tim-3 participate in the regulation of Th immune response as well as innate immunity. However, there is no report about the expression of Tim genes in Toxoplasma gondii-infected experimental models during pregnancy. In this study, Kunming outbred pregnant mice were infected with RH strain of T. gondii through vagina at days 10 to 16 of gestation, and the mRNA expressions of Tim-1, Tim-3, interleukin (IL)-4, and interferon (IFN)-γ in the placentas, uteri, and draining lumber aortic lymph nodes (LALNs) at day 18 of gestation were analyzed using quantitative real-time PCR (qRT-PCR). Compared with uninfected pregnant controls, significantly increased levels of IFN-γ and Tim-3 were detected in the placentas (P < 0.001), uteri (P = 0.003 and P = 0.017, respectively), and LALNs (P = 0.003 and P = 0.025, respectively) of T. gondii-infected mice; there were positive and significant correlations between Tim-3 and IFN-γ mRNA expression levels in the placentas (R² = 0.6331, P = 0.0011), uteri (R² = 0.5658, P = 0.003), and LALNs (R² = 0.5583, P = 0.0033) of infected mice. Tim-1 (P = 0.002) and IL-4 (P = 0.003) expressions were significantly increased in the placentas, but Tim-1 were significantly decreased in the uteri (P = 0.013) and LALNs (P < 0.001) of infected pregnant mice in comparison of uninfected pregnant controls. Our data suggested that Tim-3 may play a regulatory role in T. gondii-infected pregnant mouse model.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>25270237</pmid><doi>10.1007/s00436-014-4167-3</doi><tpages>8</tpages></addata></record> |
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| subjects | animal disease models Animals Biomedical and Life Sciences Biomedicine Female gene expression Gene Expression Regulation - physiology genes Hepatitis A Virus Cellular Receptor 2 immune response immunoglobulins Immunology innate immunity interferon-gamma Interferon-gamma - genetics Interferon-gamma - metabolism interleukin-4 lumber lymph nodes Medical Microbiology messenger RNA Mice Microbiology mucins Original Paper Pregnancy Pregnancy Complications, Parasitic - immunology Pregnancy Complications, Parasitic - metabolism quantitative polymerase chain reaction Real-Time Polymerase Chain Reaction Receptors, Virus - genetics Receptors, Virus - metabolism reverse transcriptase polymerase chain reaction Toxoplasma Toxoplasma gondii Toxoplasmosis, Animal - metabolism vagina |
| title | correlation of Tim-3 and IFN-γ expressions in mice infected with Toxoplasma gondii during gestation |
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