Detection of DNA single-strand breaks in lymphocytes of smokers

In a controlled study, ten male volunteers (five smokers and five nonsmokers) were subjected to different smoking conditions and compared to five nonsmokers, not exposed to cigarette smoke. During the 4 days of the study, nonsmoking periods were strictly controlled. On the first day the ten subjects...

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Bibliographic Details
Published in:International archives of occupational and environmental health 1993-09, Vol.65 (2), p.83-88
Main Authors: HOLZ, O, MEISSNER, R, EINHAUS, M, KOOPS, F, WARNCKE, K, SCHERER, G, ADLKOFER, F, BAUMGARTNER, E, RÜDIGER, H. W
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Carboxyhemoglobin - metabolism
Chromosome Aberrations - genetics
Cotinine - blood
DNA Damage - genetics
DNA Repair - genetics
Environmental Monitoring
Humans
Lymphocytes
Male
Medical sciences
Pesticides, fertilizers and other agrochemicals toxicology
Smoking - adverse effects
Smoking - genetics
Toxicology
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Summary:In a controlled study, ten male volunteers (five smokers and five nonsmokers) were subjected to different smoking conditions and compared to five nonsmokers, not exposed to cigarette smoke. During the 4 days of the study, nonsmoking periods were strictly controlled. On the first day the ten subjects were sham exposed. On the second day the five smokers smoked 24 cigarettes in 8 h, while the five nonsmokers were exposed to the environmental tobacco smoke. After another day of sham exposure the smoke exposure was repeated under the same conditions. Blood was drawn before and after exposure and DNA single-strand breaks (SSBs) were analyzed in lymphocytes immediately (1 h) after isolation of cells and after 4 h incubation at 37 degrees C, using a modified assay based on the nick translation reaction. Base levels of unscheduled DNA synthesis (UDS) and UDS levels were determined after 1 h incubation with methyl methanesulfonate. Duplicate analysis using the same method was performed in a second laboratory after transportation of blood samples at 0 degree C on a train from Munich to Hamburg. Tobacco smoke exposure of the subjects increased COHb and plasma cotinine levels. SSBs could be detected in all probands with some interindividual day-to-day and morning-to-evening variations. In four of five active smokers, SSB increases were found after smoking. In nonsmokers exposed to tobacco smoke no exposure-related variation in SSB levels could be detected.
ISSN:0340-0131
1432-1246
DOI:10.1007/BF00405724