Over-transcription of genes in a parathion-resistant strain of mosquito Culex pipiens quinquefasciatus

• Published in: Insect science 2015-02, Vol.22 (1), p.150-156
• Main Authors: Wang, Wei, Liu, Si‐Lu, Liu, Yang‐Yang, Qiao, Chuan‐Ling, Chen, Shao‐Liang, Cui, Feng
• Format: Article
• Language: English
• Subjects: Animals; Base Sequence; blood proteins; cDNA microarray; chymotrypsin; complementary DNA; Culex - genetics; Culex pipiens quinquefasciatus; Culex quinquefasciatus; cytochrome b; DNA, Complementary; evolutionary adaptation; Gene Expression Profiling; gene overexpression; gene up-regulation; genes; glutathione transferase; immunoblotting; insecticide resistance; Insecticide Resistance - genetics; insecticides; Insecticides - pharmacology; larvae; microarray technology; Molecular Sequence Data; Parathion - pharmacology; population size; Real-Time Polymerase Chain Reaction; ribosomal RNA; suppression subtractive hybridization; theta glutathione S-transferase; transcription (genetics); Transcription, Genetic; 化学杀虫剂; 对硫磷; 抑制性消减杂交; 抗性菌株; 致倦库蚊; 蚊子; 谷胱甘肽S-转移酶; 转录基因
• ISSN: 1672-9609; 1744-7917
• DOI: 10.1111/1744-7917.12106

Insecticide resistance is an evolutionary adaptation that develops quite quickly in mosquitoes because of the high selection pressure of chemical insecticides, rapid generation time and large population size. Identification of genes associated with insecticide resistance is fundamental to understand the complex processes responsible for resistance. We compared the gene transcriptional profiles of parathion‐resistant and ‐susceptible Culex pipiens quinquefasciatus using a combination of suppression subtractive hybridization and complementary DNA (cDNA) microarray techniques. A total of 278 colonies were selected from the resistant‐susceptible mosquito subtractive library, 38 of which showed more than two fold stronger immunoblotting signals in the resistant strain than in the susceptible strain using cDNA microarray selection. The sequencing results showed that the 38 colonies can be matched to 12 genes of C. p. quinquefasciatus. Eight genes were confirmed to be overexpressed by more than two fold in the resistant strain. These genes encode chymotrypsin‐1, theta glutathione S‐transferase, lipase 3, larval serum protein 1 β chain, cytochrome b, mitochondrial ribosomal large subunit, 28S rRNA, and a protein with unknown function. This study serves as a preliminary attempt to identify new genes associated with organophosphate resistance in this mosquito species and provides insights into the complicated physiological phenomenon of insecticide resistance.