Human cytomegalovirus replication supported by virus-induced activation of CCL2-CCR2 interactions

We previously revealed that human cytomegalovirus (HCMV) infection can cause aberrant expression of the chemokine IL-8/CXCL8. We first examined the effects of HCMV infection on the expression of another chemokine, CCL2. HCMV infection induced CCL2 expression at the mRNA and protein levels in human e...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2014-10, Vol.453 (3), p.321-325
Main Authors: Murayama, Tsugiya, Kikuchi, Marie, Miita, Takaki, Yamada, Rie, Matsubara, Keiko, Sadanari, Hidetaka, Mukaida, Naofumi
Format: Article
Language:English
Subjects:
Base Sequence
Cells, Cultured
Chemokine CCL2 - physiology
Cytomegalovirus - physiology
DNA Primers
Human cytomegalovirus
Humans
Polymerase Chain Reaction
Receptors, CCR2 - physiology
Virus Replication - physiology
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Summary:We previously revealed that human cytomegalovirus (HCMV) infection can cause aberrant expression of the chemokine IL-8/CXCL8. We first examined the effects of HCMV infection on the expression of another chemokine, CCL2. HCMV infection induced CCL2 expression at the mRNA and protein levels in human embryonic lung fibroblasts cells (HEL). Moreover, HCMV induced the mRNA expression of CCR2, a specific receptor for CCL2. CCL2 siRNA treatment reduced HCMV virion production, and this reduction was reversed by the addition of CCL2. We further observed that CCL2 siRNA, but not control siRNA, reduced the expression of HCMV immediate early gene (IE1) and HCMV UL54 gene (DNA polymerase) in a dose-dependent manner. Thus, HCMV infection is able to activate the CCL2-CCR2 interactions to further enhance HCMV infection and/or replication.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2014.09.071