Genetic analysis of the VP2-encoding gene of canine parvovirus strains from Africa

Since the emergence of canine parvovirus type-2 (CPV-2) in the early 1970s, it has been evolving into novel genetic and antigenic variants (CPV-2a, 2b and 2c) that are unevenly distributed throughout the world. Genetic characterization of CPV-2 has not been documented in Africa since 1998 apart from...

Full description

Saved in:
Bibliographic Details
Published in:Veterinary microbiology 2013-08, Vol.165 (3-4), p.460-465
Main Authors: Dogonyaro, Banenat B., Bosman, Anna-Mari, Sibeko, Kgomotso P., Venter, Estelle H., van Vuuren, Moritz
Format: Article
Language:English
Subjects:
Africa
Animals
Canine parvovirus
Canine parvoviruses
Dog Diseases - virology
Dogs
Genes, Viral - genetics
Genetic analyses
Genetic Variation
Indexing in process
Molecular Sequence Data
Parvoviridae Infections - veterinary
Parvoviridae Infections - virology
Parvovirus, Canine - classification
Parvovirus, Canine - genetics
Phylogeny
Predicted amino acid changes
VP2-encoding gene
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Since the emergence of canine parvovirus type-2 (CPV-2) in the early 1970s, it has been evolving into novel genetic and antigenic variants (CPV-2a, 2b and 2c) that are unevenly distributed throughout the world. Genetic characterization of CPV-2 has not been documented in Africa since 1998 apart from the study carried out in Tunisia 2009. A total of 139 field samples were collected from South Africa and Nigeria, detected using PCR and the full length VP2-encoding gene of 27 positive samples were sequenced and genetically analyzed. Nigerian samples (n=6), South Africa (n=19) and vaccine strains (n=2) were compared with existing sequences obtained from GenBank. The results showed the presence of both CPV-2a and 2b in South Africa and only CPV-2a in Nigeria. No CPV-2c strain was detected during this study. Phylogenetic analysis showed a clustering not strictly associated with the geographical origin of the analyzed strains, although most of the South African strains tended to cluster together and the viral strains analyzed in this study were not completely distinct from CPV-2 strains from other parts of the world. Amino acid analysis showed predicted amino acid changes.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2013.04.022