A Gene-Networked Gel Matrix-Supported Lipid Bilayer as a Synthetic Nucleus System

A spheroidal transgene-networked gel matrix was designed as a synthetic nucleus system. It was spheroidically manufactured using both advanced lithography and DNA nanotechnology. Stable Aqueorea coerulescens green fluorescent protein (AcGFP)-encoding gene cross-networks have been optimized in variou...

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Bibliographic Details
Published in:Langmuir 2012-12, Vol.28 (49), p.17036-17042
Main Authors: Bae, Sun Ju, Song, Woo Chul, Jung, Sung Hwan, Cho, Seung-Woo, Kim, Dong-Ik, Um, Soong Ho
Format: Article
Language:English
Subjects:
Cell Nucleus - chemistry
Cell Nucleus - genetics
Cell Nucleus - metabolism
Chemistry
Dimethylpolysiloxanes - chemistry
DNA, Cruciform - chemistry
DNA, Cruciform - genetics
DNA, Cruciform - metabolism
DNA, Single-Stranded - chemistry
DNA, Single-Stranded - genetics
DNA, Single-Stranded - metabolism
Escherichia coli - genetics
Exact sciences and technology
Gels - chemistry
Gels - metabolism
Gene Regulatory Networks
General and physical chemistry
Green Fluorescent Proteins
Hydrophobic and Hydrophilic Interactions
Lipid Bilayers - chemistry
Lipid Bilayers - metabolism
Models, Biological
Nucleic Acid Conformation
Plasmids
Protein Biosynthesis
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Summary:A spheroidal transgene-networked gel matrix was designed as a synthetic nucleus system. It was spheroidically manufactured using both advanced lithography and DNA nanotechnology. Stable Aqueorea coerulescens green fluorescent protein (AcGFP)-encoding gene cross-networks have been optimized in various parameters: the number of gene-networked gel (G-net-gel) spheroids, the concentration of a AcGFP plasmid in the scaffold, and the molar ratio between the X-DNA building blocks and the gene. It was then assessed that 800 units of the gene networked gel matrix at a 4000:1 molar ratio of X-DNA blocks and AcGFP gene components accomplished 20-fold enhanced in vitro protein expression efficiency for 36 h. Furthermore, once with lipid capping, it reproduced the natural nucleus system, demonstrating the 2-fold increased levels of messenger RNAs (mRNAs) relative to solution phase vectors.
ISSN:0743-7463
1520-5827
DOI:10.1021/la303498k