Urine human papillomavirus prevalence in women with high-grade cervical lesions

• Published in: European journal of obstetrics & gynecology and reproductive biology 2014-12, Vol.183, p.12-15
• Main Authors: Nicolau, P, Mancebo, G, Agramunt, S, Solé-Sedeño, J.M, Bellosillo, B, Muset, M.M, Lloveras, B, Alameda, F, Carreras, R
• Format: Article
• Language: English
• Subjects: Adult; Biopsy; Cervical cancer screening; Cervical intraepithelial dysplasia; Cervical Intraepithelial Neoplasia - epidemiology; Cervical Intraepithelial Neoplasia - pathology; Cervical Intraepithelial Neoplasia - urine; Cervix Uteri - pathology; Comorbidity; DNA, Viral - genetics; DNA, Viral - urine; DNA-HPV PCR; Female; Genotype; HPV genotypes; Human papillomavirus; Human papillomavirus 16 - genetics; Human papillomavirus 16 - isolation & purification; Humans; Middle Aged; Neoplasm Grading; Obstetrics and Gynecology; Papillomaviridae - genetics; Papillomaviridae - isolation & purification; Papillomavirus Infections - epidemiology; Papillomavirus Infections - urine; Prevalence; Retrospective Studies; Urine - virology; Urine HPV detection; Uterine Cervical Neoplasms - epidemiology; Uterine Cervical Neoplasms - pathology; Uterine Cervical Neoplasms - urine
• ISSN: 0301-2115; 1872-7654
• DOI: 10.1016/j.ejogrb.2014.10.005

Abstract Objective To determine the prevalence of human papillomavirus (HPV) in urine samples from women with high-grade cervical lesions. Secondary objectives are to identify the influence of socio-demographic factors and the different genotypes with urinary HPV positivity. Study design 75 women with a positive biopsy for CIN2+ were included in the study from October 2010 to July 2011. A sample of urine was collected immediately before conization at the outpatient clinic. We analyzed the presence of HPV using a PCR technique. Results The mean age of the patients was 34.8 years (range 24 to 61). All patients had histological CIN2+, of whom 54.67% had CIN3. The prevalence of HPV in urine test was 58.82% in CIN2 population versus 78.05% in CIN3 patients (p 0.072). 31 different genotypes were found. The most frequent HPV genotype was 16-HPV, which was identified in 58% of women with positive HPV-DNA in urine samples. No demographic characteristics were significantly associated to urinary HPV prevalence. Conclusion Most of the patients with CIN2+ showed positive results for urine HPV test. The prevalence of positive urinary HPV test was higher for patients with CIN3. HPV urine detection could be considered as an acceptable option for high-risk population who skip regular screening programs.