Increased polysaccharide production and biosynthetic gene expressions in a submerged culture of Ganoderma lucidum by the overexpression of the homologous α-phosphoglucomutase gene

This study aimed to improve the production of polysaccharide by engineering the biosynthetic pathway in Ganoderma lucidum through the overexpression of α-phosphoglucomutase (PGM) gene. PGM is responsible for the linkage between sugar catabolism and sugar anabolism. The effects of PGM gene overexpres...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2015-02, Vol.38 (2), p.399-405
Main Authors: Xu, Jun-Wei, Ji, Sen-Lin, Li, Huan-Jun, Zhou, Jiang-Sheng, Duan, Yan-Qing, Dang, Li-Zhi, Mo, Ming-He
Format: Article
Language:English
Subjects:
Bioreactors - microbiology
Biotechnology
Chemistry
Chemistry and Materials Science
Environmental Engineering/Biotechnology
Food Science
Ganoderma lucidum
Genes, Synthetic - genetics
Genetic Enhancement - methods
Homologous Recombination - genetics
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Original Paper
Phosphoglucomutase - physiology
Polysaccharides - biosynthesis
Polysaccharides - genetics
Polysaccharides - isolation & purification
Reishi - physiology
Up-Regulation - genetics
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Summary:This study aimed to improve the production of polysaccharide by engineering the biosynthetic pathway in Ganoderma lucidum through the overexpression of α-phosphoglucomutase (PGM) gene. PGM is responsible for the linkage between sugar catabolism and sugar anabolism. The effects of PGM gene overexpression on intracellular polysaccharide (IPS) content, extracellular polysaccharide (EPS) production and transcription levels of three genes encoding the enzymes involved in polysaccharide biosynthesis, including PGM, UDP-glucose pyrophosphorylase (UGP), and β-1,3-glucan synthase (GLS), were investigated. The maximum IPS content and EPS production in G. lucidum overexpressing the PGM gene were 23.67 mg/100 mg dry weight and 1.76 g/L, respectively, which were higher by 40.5 and 44.3 % than those of the wild-type strain. The transcription levels of PGM, UGP and GLS were upregulated by 4.77-, 1.51- and 1.53-fold, respectively, in the engineered strain, suggesting that increased polysaccharide biosynthesis may result from a higher expression of those genes.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-014-1279-1