Synthesis and anticervical cancer activity of novel pH responsive micelles for oral curcumin delivery

• Published in: International journal of pharmaceutics 2014-12, Vol.477 (1-2), p.261-272
• Main Authors: Sajomsang, Warayuth, Gonil, Pattarapond, Saesoo, Somsak, Ruktanonchai, Uracha Rangsadthong, Srinuanchai, Wanwisa, Puttipipatkhachorn, Satit
• Format: Article
• Language: English
• Subjects: Anticervical cancer activity; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - pharmacology; Apoptosis - drug effects; Cell Culture Techniques; Chitosan; Chitosan - analogs & derivatives; Chitosan - chemical synthesis; Chitosan - chemistry; Curcumin; Curcumin - administration & dosage; Curcumin - pharmacology; Drug Carriers - chemical synthesis; Drug Carriers - chemistry; Drug delivery system; Drug Liberation; Drug Stability; Female; HeLa Cells; Humans; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Micelles; Microscopy, Electron, Transmission; Particle Size; pH responsive micelles; Surface Properties; Surface-Active Agents - chemical synthesis; Surface-Active Agents - chemistry; Uterine Cervical Neoplasms - drug therapy; Uterine Cervical Neoplasms - pathology
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2014.10.042

[Display omitted] Curcumin (CM) has demonstrated safety and efficacy as a drug, but its pharmaceutical role is restricted as a result of extremely low aqueous solubility, rapid systemic elimination, inadequate tissue absorption and degradation at alkaline pH; properties that severely curtail its bioavailability. To address this issue, CM was encapsulated within pH responsive amphiphilic chitosan, resulting in the formation of 100nm spontaneously self-assembled polymeric micelles in water. The amphiphilic chitosan, namely N-benzyl-N,O-succinyl chitosan (BSCS), was prepared by reductive N-benzylation and N,O-succinylation. The stability of micelles after being re-dispersed in water was investigated using glycine as a cryoprotectant, and the average sizes were shown to be maintained at a level lower than 200nm for up to 4 months, at temperatures of 4°C and 25°C. In vitro drug release results showed that CM was slowly released from the micelles without any burst effect in the intestine (pH 5.5–7.4), with limited release in the stomach (pH 1.2). Cytotoxicity assays indicated that CM loaded micelles showed half maximal inhibitory concentrations (IC50) 4.7-, 3.6-, and 12.2-fold lower than that of free CM in HeLa, SiHa and C33a cervical cell lines, respectively. Cellular uptake of micelles was confirmed by confocal laser scanning microscopy and flow cytometry, with a 6-fold significant increase in the amount of CM loaded micelles compared to free CM in all cervical cancer cells. Notably, CM loaded micelles promoted an increase (30–55%) in the percentage of early apoptosis of HeLa, SiHa and C33a cells, compared to free CM. These results suggest that BSCS micelles may be a promising carrier for effective oral delivery of CM.