Apoptosis and calcification of vascular endothelial cell under hyperhomocysteinemia

In recent years, it is found that increase in Hcy level in blood can directly or indirectly cause vascular endothelial cell injury and induce vascular calcification. However, the mechanism of vascular endothelial cell injury and vascular calcification has not been studied thoroughly. This paper carr...

Full description

Saved in:
Bibliographic Details
Published in:Medical oncology (Northwood, London, England) London, England), 2015, Vol.32 (1), p.403-403, Article 403
Main Authors: Fang, Kuaifa, Chen, Zhujun, Liu, Meng, Peng, Jian, Wu, Pingsheng
Format: Article
Language:English
Subjects:
Animals
Apoptosis - physiology
Calcinosis - etiology
Calcinosis - physiopathology
Cells, Cultured
Endothelial Cells - pathology
Flow Cytometry
Hematology
Human Umbilical Vein Endothelial Cells
Humans
Hyperhomocysteinemia - complications
Internal Medicine
Medicine
Medicine & Public Health
Oncology
Original Paper
Pathology
Rats
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In recent years, it is found that increase in Hcy level in blood can directly or indirectly cause vascular endothelial cell injury and induce vascular calcification. However, the mechanism of vascular endothelial cell injury and vascular calcification has not been studied thoroughly. This paper carried out experiment for research aiming at discussing the effect and action mechanism of Hhcy on endothelial cells and vascular calcification. Firstly, human umbilical vein endothelial cells (HUVECs) were cultured and then intervened by Hcy of different concentrations (0, 0.01, 0.1, 1.0, 3.0, 5.0 mmol/L) and at different action time (3, 6, 12, 24 h). Then apoptosis rate and reactive oxygen were detected by flow cytometry. At the same time, the model for the culture of rat vascular calcification was set up and induced into Hhcy so as to detect the total plasma Hcy level and judge vascular calcification degree. The results showed that with the increase in Hcy concentration and extension of action period, the apoptosis rate and generation of reactive oxygen of HUVECs all significantly increased, and the differences were all statistically significant ( P  
ISSN:1357-0560
1559-131X
DOI:10.1007/s12032-014-0403-z