Novel compounds that enhance Agrobacterium-mediated plant transformation by mitigating oxidative stress

• Published in: Plant cell reports 2015-02, Vol.34 (2), p.291-309
• Main Authors: Dan, Yinghui, Zhang, Song, Zhong, Heng, Yi, Hochul, Sainz, Manuel B
• Format: Article
• Language: English
• Subjects: Agricultural biotechnology; Agrobacterium radiobacter; Agrobacterium tumefaciens; Agrobacterium tumefaciens - drug effects; Agrobacterium tumefaciens - genetics; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Biomedical and Life Sciences; Biotechnology; Cell Biology; cell death; Cell Death - drug effects; DNA, Bacterial; genetic transformation; Glucuronidase; Glycine max - drug effects; Glycine max - genetics; Life Sciences; Limiting factors; lipoic acid; Lycopersicon esculentum; melatonin; Melatonin - chemistry; Melatonin - pharmacology; monitoring; Mortality; Original Paper; Oxidative stress; Oxidative Stress - drug effects; Plant Biochemistry; Plant Sciences; Plants, Genetically Modified - drug effects; screening; Solanum lycopersicum - drug effects; Solanum lycopersicum - genetics; Southern blotting; Soybeans; Tomatoes; transfer DNA; Transformation, Genetic; Transgenes; Transgenic plants
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-014-1707-3

KEY MESSAGE : Agrobacterium tumefaciens caused tissue browning leading to subsequent cell death in plant transformation and novel anti-oxidative compounds enhanced Agrobacterium -mediated plant transformation by mitigating oxidative stress. Browning and death of cells transformed with Agrobacterium tumefaciens is a long-standing and high impact problem in plant transformation and the agricultural biotechnology industry, severely limiting the production of transgenic plants. Using our tomato cv. MicroTom transformation system, we demonstrated that Agrobacterium caused tissue browning (TB) leading to subsequent cell death by our correlation study. Without an antioxidant (lipoic acid, LA) TB was severe and associated with high levels of GUS transient expression and low stable transformation frequency (STF). LA addition shifted the curve in that most TB was intermediate and associated with the highest levels of GUS transient expression and STF. We evaluated 18 novel anti-oxidative compounds for their potential to enhance Agrobacterium-mediated transformation, by screening for TB reduction and monitoring GUS transient expression. Promising compounds were further evaluated for their effect on MicroTom and soybean STF. Among twelve non-antioxidant compounds, seven and five significantly (P