Long-term Sequential Changes of Radiation Proctitis and Angiopathy in Rats

The purpose of the present study was to establish an experimental rat model for late radiation proctitis, and to examine the assessment strategy for late radiation proctitis. A total of 57 Wistar rats were used. Fourty-five of the rats were exposed to selective rectal irradiation with a single fract...

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Bibliographic Details
Published in:JOURNAL OF RADIATION RESEARCH 2012, Vol.53 (2), p.217-224
Main Authors: Doi, Hiroshi, Kamikonya, Norihiko, Takada, Yasuhiro, Fujiwara, Masayuki, Tsuboi, Keita, Miura, Hideharu, Inoue, Hiroyuki, Tanooka, Masao, Nakamura, Takeshi, Shikata, Toshiyuki, Kimura, Takeshi, Tsujimura, Tohru, Hirota, Shozo
Format: Article
Language:English
Subjects:
Animals
Disease Models, Animal
Female
Humans
Longitudinal Studies
Peripheral Arterial Disease - pathology
Proctitis
Proctitis - pathology
Radiation (Physics)
Radiation Injuries - pathology
Radiation Injuries, Experimental - pathology
Rats
Rats, Wistar
Rectum - pathology
Rectum - radiation effects
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Summary:The purpose of the present study was to establish an experimental rat model for late radiation proctitis, and to examine the assessment strategy for late radiation proctitis. A total of 57 Wistar rats were used. Fourty-five of the rats were exposed to selective rectal irradiation with a single fraction of 25 Gy. These rats were sacrificed at the 4th, 12th, 24th, and 37th week following irradiation. The remaining 12 rats comprised the control group without irradiation. The rectal mucosa of each rat was evaluated macroscopically and pathologically. The number of vessels in the rectal mucosa was counted microscopically. In addition, the vascular stenosis was evaluated. In the results, the degree of clinical and macroscopic findings decreased following acute proctitis and developed later. In the pathological examination, mucosal changes and microangiopathy were followed up, as well. The absolute number of vessels in the rectum was the greatest at the 12th week following irradiation and was the lowest in the control group. The severity of the microangiopathy was also well evaluated. To conclude, we established an animal experimental model of late radiation proctitis, and also established an assessment strategy to evaluate objectively the severity of late radiation proctitis with focusing on microangiopathy using an animal experimental model. This model can be used as an animal experimental model of radiation-induced microangiopathy.
ISSN:0449-3060
1349-9157
DOI:10.1269/jrr.11075