5-HT3 receptor expression in the mouse vestibular ganglion

Abstract The 5-hydroxytryptamine type 3 (5-HT3 ) receptor is a ligand-gated ion channel and a member of the Cys-loop family of receptors. Previous studies have shown 5-HT3 receptor expression in various neural cells of the central and peripheral nervous systems. Although the function and distributio...

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Bibliographic Details
Published in:Brain research 2014-04, Vol.1557, p.74-82
Main Authors: Takimoto, Yasumitsu, Ishida, Yusuke, Nakamura, Yukiko, Kamakura, Takefumi, Yamada, Takahiro, Kondo, Makoto, Kitahara, Tadashi, Uno, Atsuhiko, Imai, Takao, Horii, Arata, Okazaki, Suzuyo, Nishiike, Suetaka, Inohara, Hidenori, Shimada, Shoichi
Format: Article
Language:English
Subjects:
5-HT
Biological and medical sciences
Cochlea
Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation
Fundamental and applied biological sciences. Psychology
HTR3
In situ hybridization
Inner ear
Neurology
Spiral ganglion
Vertebrates: nervous system and sense organs
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Summary:Abstract The 5-hydroxytryptamine type 3 (5-HT3 ) receptor is a ligand-gated ion channel and a member of the Cys-loop family of receptors. Previous studies have shown 5-HT3 receptor expression in various neural cells of the central and peripheral nervous systems. Although the function and distribution of the 5-HT3 receptor has been well established, its role in the inner ear is still poorly understood. Moreover, no study has yet determined its localization and function in the peripheral vestibular nervous system. In the present study, we reveal mRNA expression of both 5-HT3A and 5-HT3B receptor subunits in the mouse vestibular ganglion (VG) by RT-PCR and in situ hybridization (ISH). We also show by ISH that 5-HT3 receptor mRNA is only expressed in the VG (superior and inferior division) in the peripheral vestibular nervous system. Moreover, we performed Ca2+ imaging to determine whether functional 5-HT3 receptors are present in the mouse VG, using a selective 5-HT3 receptor agonist, SR57227A. In wild mice, 32% of VG neurons responded to the agonist, whereas there was no response in 5-HT3A receptor knockout mice. These results indicate that VG cells express functional 5-HT3 receptor channels and might play a modulatory role in the peripheral vestibular nervous system.
ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2014.02.016