Gene expression profiling of rootstock ‘140Ru’ and Vitis vinifera L. cv. ‘Crimson Seedless’ grape roots infected with grape phylloxera

• Published in: Plant growth regulation 2014-05, Vol.73 (1), p.1-8
• Main Authors: Du, Yuan-Peng, Jiang, En-Shun, Wang, Feng-Pan, Zhang, Shi-Zhong, Zhai, Heng
• Format: Article
• Language: English
• Subjects: Agriculture; Biomedical and Life Sciences; Biosynthesis; carbohydrate metabolism; Daktulosphaira vitifolia; Daktulosphaira vitifoliae; energy metabolism; Fruits; gene expression; genes; grapes; Life Sciences; Management tools; Metabolites; Original Paper; Pests; Plant Anatomy/Development; Plant Physiology; Plant Sciences; reverse transcriptase polymerase chain reaction; Roots; rootstocks; Secondary metabolites; Vitaceae; Vitis vinifera
• ISSN: 0167-6903; 1573-5087
• DOI: 10.1007/s10725-013-9862-z

Grape phylloxera (Daktulosphaira vitifolia Fitch) is the most devastating pest of grape. Information concerning the molecular basis of grape root–phylloxera interactions will enhance the future development of effective management tools against this pest. In this study, changes in gene transcript abundance in grape roots in response to phylloxera infestation were measured using the Affymetrix Vitis vinifera Genome Array. Two genetically related grape roots, rootstock ‘140Ru’ (potted ‘140Ru’, which forms nodosities but no tuberosities in response to phylloxera infestation) and V. vinifera L. cv. ‘Crimson Seedless’ (which forms both nodosities and tuberosities) were utilized in the current study. Pairwise comparisons followed by false discovery rate analysis indicated that the expression levels of 1,204 transcripts changed significantly in ‘140Ru’; 599 increased and 625 decreased. However, in ‘Crimson Seedless’, the expression of 799 transcripts changed significantly, including 305 that increased and 494 that decreased. Comparative analysis of these transcripts indicated that a total of 155 transcripts were common to both resistance and susceptible responses. In this set, the levels of 48 transcripts increased in ‘140Ru’ but decreased in ‘Crimson Seedless’. Quantitative real-time RT-PCR confirmed the results of the microarray analysis. Since nodosities potentially function as nutrient reservoirs, probe sets corresponding to the categories of energy metabolism and carbohydrate metabolism were more commonly induced in ‘Crimson Seedless’. Defense responses of ‘140Ru’ to phylloxera attack were detected. Probe sets that were uniquely induced in ‘140Ru’ are associated with the biosynthesis of secondary metabolites. This study may aid in the identification of genes involved in plant defenses against grape phylloxera.