Complete genome sequence of motherwort yellow mottle virus, a novel putative member of the genus Torradovirus

• Published in: Archives of virology 2015-02, Vol.160 (2), p.587-590
• Main Authors: Seo, Jang-Kyun, Kang, Minji, Kwak, Hae-Ryun, Kim, Mi-Kyeong, Kim, Chang-Seok, Lee, Su-Heon, Kim, Jeong-Soo, Choi, Hong-Soo
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Amino acids; Annotated Sequence Record; Base Sequence; Biomedical and Life Sciences; Biomedicine; Genome, Viral - genetics; Genomes; High-Throughput Nucleotide Sequencing; Infectious Diseases; Leonurus - virology; Lettuce; Medical Microbiology; Open Reading Frames - genetics; Phylogenetics; Phylogeny; Plant Diseases - virology; Plant Viruses - classification; Plant Viruses - genetics; Proteins; RNA Viruses - classification; RNA Viruses - genetics; RNA, Viral - genetics; Sequence Analysis, RNA; Sequence Homology, Amino Acid; Taxonomy; Viral Proteins - genetics; Virology; Viruses
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-014-2254-5

The complete genome sequence of a new virus isolated from a motherwort plant exhibiting yellow mottle, mild mosaic, and stunting symptoms in Andong, Korea, was determined. The genome of this virus is composed of two single-stranded RNAs (7068 and 4963 nucleotides in length, respectively) carrying poly(A) tails. RNA1 contains one large open reading frame (RNA1-ORF1), while two potential ORFs (RNA2-ORF1 and RNA2-ORF2) were found in RNA2. BLAST searches of protein databases showed that RNA1-ORF1 and RNA2-ORF2 have maximum amino acid sequence identities of 53 % and 57 % to the RNA1-ORF1 and RNA2-ORF2, respectively, of lettuce necrotic leaf curl virus (LNLCV, a recently identified torradovirus). Phylogenetic analysis provided further evidence that the virus identified in this study is probably a member of a new species in the genus Torradovirus . The name “motherwort yellow mottle virus” (MYMoV) is proposed for this new virus.