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The CD8α gene in duck (Anatidae): cloning, characterization, and expression during viral infection
Cluster of differentiation 8 alpha (CD8α) is critical for cell-mediated immune defense and T-cell development. Although CD8α sequences have been reported for several species, very little is known about CD8α in ducks. To elucidate the mechanisms involved in the innate and adaptive immune responses of...
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Published in: | Molecular biology reports 2015-02, Vol.42 (2), p.431-439 |
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container_title | Molecular biology reports |
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creator | Xu, Qi Chen, Yang Zhao, Wen Ming Huang, Zheng Yang Duan, Xiu Jun Tong, Yi Yu Zhang, Yang Li, Xiu Chang, Guo Bin Chen, Guo Hong |
description | Cluster of differentiation 8 alpha (CD8α) is critical for cell-mediated immune defense and T-cell development. Although
CD8α
sequences have been reported for several species, very little is known about
CD8α
in ducks. To elucidate the mechanisms involved in the innate and adaptive immune responses of ducks, we cloned
CD8α
coding sequences from domestic, Muscovy, Mallard, and Spotbill ducks using reverse transcription polymerase chain reaction (RT-PCR). Each sequence consisted of 714 nucleotides and encoded a signal peptide, an IgV-like domain, a stalk region, a transmembrane region, and a cytoplasmic tail. We identified 58 nucleotide differences and 37 amino acid differences among the four types of duck; of these, 53 nucleotide and 33 amino acid differences were between Muscovy ducks and the other duck species. The
CD8α
cDNA sequence from domestic duck consisted of a 61-nucleotide 5′ untranslated region (UTR), a 714-nucleotide open reading frame, and an 849-nucleotide 3′ UTR. Multiple sequence alignments showed that the amino acid sequence of CD8α is conserved in vertebrates. RT-PCR revealed that expression of
CD8α
mRNA of domestic ducks was highest in the thymus and very low in the kidney, cerebrum, cerebellum, and muscle. Immunohistochemical analyses detected CD8α on the splenic corpuscle and periarterial lymphatic sheath of the spleen.
CD8α
mRNA in domestic ducklings was initially up-regulated, and then down-regulated, in the thymus, spleen, and liver after treatment with duck hepatitis virus type I (DHV-1) or the immunostimulant polyriboinosinic polyribocytidylic acid (poly I:C). |
doi_str_mv | 10.1007/s11033-014-3784-3 |
format | article |
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CD8α
sequences have been reported for several species, very little is known about
CD8α
in ducks. To elucidate the mechanisms involved in the innate and adaptive immune responses of ducks, we cloned
CD8α
coding sequences from domestic, Muscovy, Mallard, and Spotbill ducks using reverse transcription polymerase chain reaction (RT-PCR). Each sequence consisted of 714 nucleotides and encoded a signal peptide, an IgV-like domain, a stalk region, a transmembrane region, and a cytoplasmic tail. We identified 58 nucleotide differences and 37 amino acid differences among the four types of duck; of these, 53 nucleotide and 33 amino acid differences were between Muscovy ducks and the other duck species. The
CD8α
cDNA sequence from domestic duck consisted of a 61-nucleotide 5′ untranslated region (UTR), a 714-nucleotide open reading frame, and an 849-nucleotide 3′ UTR. Multiple sequence alignments showed that the amino acid sequence of CD8α is conserved in vertebrates. RT-PCR revealed that expression of
CD8α
mRNA of domestic ducks was highest in the thymus and very low in the kidney, cerebrum, cerebellum, and muscle. Immunohistochemical analyses detected CD8α on the splenic corpuscle and periarterial lymphatic sheath of the spleen.
CD8α
mRNA in domestic ducklings was initially up-regulated, and then down-regulated, in the thymus, spleen, and liver after treatment with duck hepatitis virus type I (DHV-1) or the immunostimulant polyriboinosinic polyribocytidylic acid (poly I:C).</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-014-3784-3</identifier><identifier>PMID: 25332128</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Amino Acid Sequence ; Anatidae ; Animal Anatomy ; Animal Biochemistry ; Animals ; Biomedical and Life Sciences ; CD8 Antigens - genetics ; CD8 Antigens - metabolism ; Cloning, Molecular ; DNA, Complementary ; Ducks - genetics ; Ducks - virology ; Gene Expression ; Hepatitis Virus, Duck ; Histology ; Life Sciences ; Morphology ; Organ Specificity - genetics ; Phylogeny ; RNA, Messenger - genetics ; Sequence Alignment ; Sequence Analysis, DNA</subject><ispartof>Molecular biology reports, 2015-02, Vol.42 (2), p.431-439</ispartof><rights>Springer Science+Business Media Dordrecht 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-1b091db820bd8532124b5feaf437882eba56f4fab7d2b5feff97f568af32ce83</citedby><cites>FETCH-LOGICAL-c447t-1b091db820bd8532124b5feaf437882eba56f4fab7d2b5feff97f568af32ce83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25332128$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Qi</creatorcontrib><creatorcontrib>Chen, Yang</creatorcontrib><creatorcontrib>Zhao, Wen Ming</creatorcontrib><creatorcontrib>Huang, Zheng Yang</creatorcontrib><creatorcontrib>Duan, Xiu Jun</creatorcontrib><creatorcontrib>Tong, Yi Yu</creatorcontrib><creatorcontrib>Zhang, Yang</creatorcontrib><creatorcontrib>Li, Xiu</creatorcontrib><creatorcontrib>Chang, Guo Bin</creatorcontrib><creatorcontrib>Chen, Guo Hong</creatorcontrib><title>The CD8α gene in duck (Anatidae): cloning, characterization, and expression during viral infection</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Cluster of differentiation 8 alpha (CD8α) is critical for cell-mediated immune defense and T-cell development. Although
CD8α
sequences have been reported for several species, very little is known about
CD8α
in ducks. To elucidate the mechanisms involved in the innate and adaptive immune responses of ducks, we cloned
CD8α
coding sequences from domestic, Muscovy, Mallard, and Spotbill ducks using reverse transcription polymerase chain reaction (RT-PCR). Each sequence consisted of 714 nucleotides and encoded a signal peptide, an IgV-like domain, a stalk region, a transmembrane region, and a cytoplasmic tail. We identified 58 nucleotide differences and 37 amino acid differences among the four types of duck; of these, 53 nucleotide and 33 amino acid differences were between Muscovy ducks and the other duck species. The
CD8α
cDNA sequence from domestic duck consisted of a 61-nucleotide 5′ untranslated region (UTR), a 714-nucleotide open reading frame, and an 849-nucleotide 3′ UTR. Multiple sequence alignments showed that the amino acid sequence of CD8α is conserved in vertebrates. RT-PCR revealed that expression of
CD8α
mRNA of domestic ducks was highest in the thymus and very low in the kidney, cerebrum, cerebellum, and muscle. Immunohistochemical analyses detected CD8α on the splenic corpuscle and periarterial lymphatic sheath of the spleen.
CD8α
mRNA in domestic ducklings was initially up-regulated, and then down-regulated, in the thymus, spleen, and liver after treatment with duck hepatitis virus type I (DHV-1) or the immunostimulant polyriboinosinic polyribocytidylic acid (poly I:C).</description><subject>Amino Acid Sequence</subject><subject>Anatidae</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>CD8 Antigens - genetics</subject><subject>CD8 Antigens - metabolism</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary</subject><subject>Ducks - genetics</subject><subject>Ducks - virology</subject><subject>Gene Expression</subject><subject>Hepatitis Virus, Duck</subject><subject>Histology</subject><subject>Life Sciences</subject><subject>Morphology</subject><subject>Organ Specificity - genetics</subject><subject>Phylogeny</subject><subject>RNA, Messenger - genetics</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkc1OGzEUha2Kqgm0D9AN8jJIGerfsYddFKBFitRN9pbHcx0GJp7UzqC2b9UX4ZnwKIFlxcaWdb9z5HsOQl8puaSEqG-JUsJ5QagouNL5-ICmVCpeiErpEzQlnNBCaEkn6DSlB0KIoEp-QhMmOWeU6Sly63vAy2v9_A9vIABuA24G94hni2D3bWPh4gq7rg9t2Myxu7fRuj3E9m8e9mGObWgw_N5FSCm_szRmED-10XbZyoMbsc_oo7ddgi_H-wytb2_Wyx_F6uf3u-ViVTgh1L6gNaloU2tG6kbL8X-ilh6sF3k5zaC2svTC21o1bBx4XykvS209Zw40P0Ozg-0u9r8GSHuzbZODrrMB-iEZWpZ5_6qs5DtQyQTTjKuM0gPqYp9SBG92sd3a-MdQYsYWzKEFk1swYwuGZ8350X6ot9C8KV5jzwA7AGk3BgbRPPRDDDmc_7i-AHB4kqU</recordid><startdate>20150201</startdate><enddate>20150201</enddate><creator>Xu, Qi</creator><creator>Chen, Yang</creator><creator>Zhao, Wen Ming</creator><creator>Huang, Zheng Yang</creator><creator>Duan, Xiu Jun</creator><creator>Tong, Yi Yu</creator><creator>Zhang, Yang</creator><creator>Li, Xiu</creator><creator>Chang, Guo Bin</creator><creator>Chen, Guo Hong</creator><general>Springer Netherlands</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20150201</creationdate><title>The CD8α gene in duck (Anatidae): cloning, characterization, and expression during viral infection</title><author>Xu, Qi ; Chen, Yang ; Zhao, Wen Ming ; Huang, Zheng Yang ; Duan, Xiu Jun ; Tong, Yi Yu ; Zhang, Yang ; Li, Xiu ; Chang, Guo Bin ; Chen, Guo Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-1b091db820bd8532124b5feaf437882eba56f4fab7d2b5feff97f568af32ce83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Amino Acid Sequence</topic><topic>Anatidae</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>CD8 Antigens - genetics</topic><topic>CD8 Antigens - metabolism</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary</topic><topic>Ducks - genetics</topic><topic>Ducks - virology</topic><topic>Gene Expression</topic><topic>Hepatitis Virus, Duck</topic><topic>Histology</topic><topic>Life Sciences</topic><topic>Morphology</topic><topic>Organ Specificity - genetics</topic><topic>Phylogeny</topic><topic>RNA, Messenger - genetics</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Qi</creatorcontrib><creatorcontrib>Chen, Yang</creatorcontrib><creatorcontrib>Zhao, Wen Ming</creatorcontrib><creatorcontrib>Huang, Zheng Yang</creatorcontrib><creatorcontrib>Duan, Xiu Jun</creatorcontrib><creatorcontrib>Tong, Yi Yu</creatorcontrib><creatorcontrib>Zhang, Yang</creatorcontrib><creatorcontrib>Li, Xiu</creatorcontrib><creatorcontrib>Chang, Guo Bin</creatorcontrib><creatorcontrib>Chen, Guo Hong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Qi</au><au>Chen, Yang</au><au>Zhao, Wen Ming</au><au>Huang, Zheng Yang</au><au>Duan, Xiu Jun</au><au>Tong, Yi Yu</au><au>Zhang, Yang</au><au>Li, Xiu</au><au>Chang, Guo Bin</au><au>Chen, Guo Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The CD8α gene in duck (Anatidae): cloning, characterization, and expression during viral infection</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2015-02-01</date><risdate>2015</risdate><volume>42</volume><issue>2</issue><spage>431</spage><epage>439</epage><pages>431-439</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Cluster of differentiation 8 alpha (CD8α) is critical for cell-mediated immune defense and T-cell development. Although
CD8α
sequences have been reported for several species, very little is known about
CD8α
in ducks. To elucidate the mechanisms involved in the innate and adaptive immune responses of ducks, we cloned
CD8α
coding sequences from domestic, Muscovy, Mallard, and Spotbill ducks using reverse transcription polymerase chain reaction (RT-PCR). Each sequence consisted of 714 nucleotides and encoded a signal peptide, an IgV-like domain, a stalk region, a transmembrane region, and a cytoplasmic tail. We identified 58 nucleotide differences and 37 amino acid differences among the four types of duck; of these, 53 nucleotide and 33 amino acid differences were between Muscovy ducks and the other duck species. The
CD8α
cDNA sequence from domestic duck consisted of a 61-nucleotide 5′ untranslated region (UTR), a 714-nucleotide open reading frame, and an 849-nucleotide 3′ UTR. Multiple sequence alignments showed that the amino acid sequence of CD8α is conserved in vertebrates. RT-PCR revealed that expression of
CD8α
mRNA of domestic ducks was highest in the thymus and very low in the kidney, cerebrum, cerebellum, and muscle. Immunohistochemical analyses detected CD8α on the splenic corpuscle and periarterial lymphatic sheath of the spleen.
CD8α
mRNA in domestic ducklings was initially up-regulated, and then down-regulated, in the thymus, spleen, and liver after treatment with duck hepatitis virus type I (DHV-1) or the immunostimulant polyriboinosinic polyribocytidylic acid (poly I:C).</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>25332128</pmid><doi>10.1007/s11033-014-3784-3</doi><tpages>9</tpages></addata></record> |
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source | Springer Nature |
subjects | Amino Acid Sequence Anatidae Animal Anatomy Animal Biochemistry Animals Biomedical and Life Sciences CD8 Antigens - genetics CD8 Antigens - metabolism Cloning, Molecular DNA, Complementary Ducks - genetics Ducks - virology Gene Expression Hepatitis Virus, Duck Histology Life Sciences Morphology Organ Specificity - genetics Phylogeny RNA, Messenger - genetics Sequence Alignment Sequence Analysis, DNA |
title | The CD8α gene in duck (Anatidae): cloning, characterization, and expression during viral infection |
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