Intestinal epithelial cells promote secretion of leptin and adiponectin in adipocytes

Although leptin and adiponectin are the predominant adipokines, how their circulating levels are regulated is incompletely understood. The present study tested whether intestinal epithelial cells influence the expression and secretion of these adipokines by adipocytes. Leptin gene expression and sec...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2015-03, Vol.458 (2), p.362-368
Main Authors: Ishihara, Rino, Mizuno, Yuki, Miwa, Akiho, Hamada, Akihiro, Tsuruta, Takeshi, Wabitsch, Martin, Sonoyama, Kei
Format: Article
Language:English
Subjects:
Adipocyte
Adipocytes - secretion
Adiponectin
Adiponectin - secretion
Animals
Caco-2 Cells
Cell Communication - physiology
Epithelial Cells - cytology
Epithelial Cells - secretion
Humans
Ileum - cytology
Ileum - secretion
Intestinal Mucosa - cytology
Intestinal Mucosa - secretion
Intestine
Leptin
Leptin - secretion
Mice
Mice, Inbred C57BL
Up-Regulation - physiology
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Summary:Although leptin and adiponectin are the predominant adipokines, how their circulating levels are regulated is incompletely understood. The present study tested whether intestinal epithelial cells influence the expression and secretion of these adipokines by adipocytes. Leptin gene expression and secretion by cultured human primary adipocytes and Simpson–Golabi–Behmel Syndrome adipocytes increased upon coculture with human enterocytic Caco-2 cells or incubation in conditioned medium of Caco-2 cells. Although adiponectin secretion increased, its mRNA levels decreased. Tissue homogenate of the ileum (but not the jejunum, colon, or liver) of nonobese C57BL/6J mice also stimulated leptin and adiponectin secretion by cultured murine 3T3-L1 adipocytes. However, ileal homogenate of obese KK-Ay mice had no effect on leptin and adiponectin secretion. We propose that as yet unidentified humoral factors released from intestinal epithelial cells are involved in regulating circulating leptin and adiponectin levels. Decreased production of such factors may contribute to hyperphagia in KK-Ay mice. •How leptin expression and secretion are regulated is incompletely understood.•Coculture with Caco-2 cells promoted leptin and adiponectin secretion.•Murine ileal homogenate stimulated leptin and adiponectin secretion.•Ileal homogenate of obese mice failed to stimulate leptin and adiponectin secretion.•We propose that ileum-derived factors regulate circulating leptin and adiponectin.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2015.01.118