Comparison of enzyme-linked immunosorbent assay and high-performance liquid chromatography for the analysis of atrazine in water from Czechoslovakia

Twenty-two water samples from Bohemian Paradise in Czechoslovakia were analyzed for atrazine (2-chloro-4-ethylamino-6- isopropylamino-1,3,5-triazine) by enzyme immunoassay (EIA) and high-performance liquid chromatography (HPLC). Three samples were drinking water, the others were creek water. The amo...

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Bibliographic Details
Published in:Archives of environmental contamination and toxicology 1991-09, Vol.21 (3), p.365-370
Main Authors: Bushway, R.J. (University of Maine, Orono, ME), Perkins, L.B, Fukal, L, Harrison, R.O, Ferguson, B.S
Format: Article
Language:English
Subjects:
ACCURACY
Analysis methods
ANALYTICAL METHODS
Applied sciences
ATRAZINA
ATRAZINE
Atrazine - analysis
CHROMATOGRAPHIE LIQUIDE HAUTE PRESS
Chromatography, High Pressure Liquid
CROMATOGRAFIA LIQUIDA ALTA PRESION
CSFR
Czechoslovakia
ECHANTILLONNAGE
Enzyme-Linked Immunosorbent Assay
Exact sciences and technology
Fresh Water - analysis
Freshwater
HIGH PRESSURE LIQUID CHROMATOGRAPHY
HPLC
MUESTREO
Natural water pollution
Pollution
POLLUTION DE L'EAU
POLUCION DEL AGUA
Reproducibility of Results
RFCS
RFTS
SAMPLING
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
Water Pollutants, Chemical - analysis
WATER POLLUTION
Water Supply - analysis
Water treatment and pollution
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Summary:Twenty-two water samples from Bohemian Paradise in Czechoslovakia were analyzed for atrazine (2-chloro-4-ethylamino-6- isopropylamino-1,3,5-triazine) by enzyme immunoassay (EIA) and high-performance liquid chromatography (HPLC). Three samples were drinking water, the others were creek water. The amount of atrazine found by EIA ranged from 0.2 to 12,700 ng/ml and 0.10 to 10,300 ng/ml by HPLC. Agreement between the methods was good. Nine samples containing the highest concentrations of atrazine were used to check the day-to-day reproducibility of both methods at high atrazine levels. The percent coefficients of variation (% CVs) ranged from 5.9 to 26.2 for the EIA and 1.5 to 33.2 for the HPLC. Day to day % CV values for each calibrator concentration ranged from 5.7 to 10.9 for EIA and 9.9 to 13.9 for HPLC. Sensitivity of the EIA was 0.2 ng/ml while it was 0.1 ng/ml for solid phase extraction HPLC and slightly less than 60 ng/ml for direct injection HPLC.
ISSN:0090-4341
1432-0703
DOI:10.1007/bf01060357