Removal of nitrogen by heterotrophic nitrification–aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24

•P. stutzeri YG-24 simultaneously remove nitrogen and phosphorus.•High total nitrogen and ammonium removal capacity in wastewater samples.•The NH4+-N→NO2−-N→gas pathway was detected. Phosphate accumulating bacterium Pseudomonas stutzeri YG-24 exhibited efficient heterotrophic nitrification and aerob...

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Bibliographic Details
Published in:Bioresource technology 2015-04, Vol.182, p.18-25
Main Authors: Li, Chune, Yang, Jinshui, Wang, Xin, Wang, Entao, Li, Baozhen, He, Ruoxue, Yuan, Hongli
Format: Article
Language:English
Subjects:
Aerobic denitrification
Aerobiosis
Ammonia - metabolism
Denitrification
Heterotrophic nitrification
Heterotrophic Processes
Nitrification
Nitrogen - metabolism
Nitrogen removal
Phosphate accumulating bacterium
Phosphates - metabolism
Phylogeny
Pseudomonas stutzeri - genetics
Pseudomonas stutzeri - metabolism
Pseudomonas stutzeri YG-24
Real-Time Polymerase Chain Reaction
Waste Disposal, Fluid - methods
Waste Water
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Summary:•P. stutzeri YG-24 simultaneously remove nitrogen and phosphorus.•High total nitrogen and ammonium removal capacity in wastewater samples.•The NH4+-N→NO2−-N→gas pathway was detected. Phosphate accumulating bacterium Pseudomonas stutzeri YG-24 exhibited efficient heterotrophic nitrification and aerobic denitrification ability. Single factor experiments showed that both heterotrophic nitrification and aerobic denitrification occurred with sodium citrate as carbon source and lower C/N ratio of 8. High average NH4+-N, NO2−-N and NO3−-N removal rates of 8.75, 7.51 and 7.73mgL−1h−1 were achieved. The application of strain YG-24 in wastewater samples resulted in TN, NH4+-N, NO2−-N, NO3−-N and P removal efficiencies of 85.28%, 88.13%, 86.15%, 70.83% and 51.21%. Sequencing and quantitative amplification by real-time PCR of napA, nirS and ppk showed that nitrogen removal pathway of strain YG-24 was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification (NH4+-N to NO2−-N and then to gaseous nitrogen) directly. These results demonstrated the strain as a suitable candidate to simultaneously remove both nitrogen and phosphate in wastewater treatment.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2015.01.100