Effects of resveratrol on doxorubicin induced testicular damage in rats

• Published in: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2015-03, Vol.67 (3), p.229-235
• Main Authors: Türedi, Sibel, Yuluğ, Esin, Alver, Ahmet, Kutlu, Ömer, Kahraman, Cemil
• Format: Article
• Language: English
• Subjects: Animals; Antibiotics, Antineoplastic - toxicity; Antioxidants - pharmacology; Apoptosis; Apoptosis - drug effects; Disease Models, Animal; Doxorubicin; Doxorubicin - toxicity; In Situ Nick-End Labeling; Infertility; Male; Rats; Rats, Sprague-Dawley; Resveratrol; Sperm Motility - drug effects; Spermatozoa - drug effects; Spermatozoa - pathology; Stilbenes - pharmacology; Testicular toxicity; Testis - drug effects; Testis - pathology
• ISSN: 0940-2993; 1618-1433
• DOI: 10.1016/j.etp.2014.12.002

The purpose of this study was to evaluate the likely protective effect of resveratrol (RES) on doxorubicin (DOX) induced testicular damage. Rats were divided into five groups: control, RES, dimethyl sulfoxide (DMSO), DOX and DOX+RES. At the end of treatment, the rats were sacrificed. Plasma testosterone levels, oxidative status, epididymal sperm parameters and testicular apoptosis were evaluated. MDA levels, GP-x and GSH activities were higher in the DOX group than in the control group. MDA levels were lower in the DOX+RES group than in the DOX group. The DOX group exhibited a significant decrease in plasma testosterone levels, sperm concentration and motility, and a significant increase in abnormal sperm rate and TUNEL (+) cells in the testis. A significant increase was observed in plasma testosterone levels and sperm concentration and motility, and a significant decrease in the abnormal sperm rate and TUNEL (+) cells in the DOX+RES group compared to the DOX group. A marked improvement in severe degenerative alterations in the germinative epithelium was also observed following treatment with RES. In conclusion, RES makes a positive contribution to fertility by exhibiting anti-apoptotic and antiperoxidative effects against DOX-induced testicular damage.