Cotton Major Latex Protein 28 Functions as a Positive Regulator of the Ethylene Responsive Factor 6 in Defense against Verticillium dahliae

• Published in: Molecular plant 2015-03, Vol.8 (3), p.399-411
• Main Authors: Yang, Chun-Lin, Liang, Shan, Wang, Hai-Yun, Han, Li-Bo, Wang, Fu-Xin, Cheng, Huan-Qing, Wu, Xiao-Min, Qu, Zhan-Liang, Wu, Jia-He, Xia, Gui-Xian
• Format: Article
• Language: English
• Subjects: cotton; Disease Resistance; disease tolerance; ethylene responsive factor; Ethylenes - metabolism; Gene Expression Regulation, Plant; Gossypium - genetics; Gossypium - immunology; Gossypium - microbiology; interaction; major latex protein; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Proteins - genetics; Plant Proteins - immunology; Salicylic Acid - metabolism; Verticillium - physiology; 乙烯; 响应因子; 基因沉默; 棉花植株; 胶蛋白; 转基因植物; 防御; 黄萎病
• ISSN: 1674-2052; 1752-9867
• DOI: 10.1016/j.molp.2014.11.023

In this study, we identified a defense-related major latex protein （MLP） from upland cotton （designated GhMLP28） and investigated its functional mechanism. GhMLP28 transcripts were ubiquitously present in cotton plants, with higher accumulation in the root. Expression of the GhMLP28 gene was induced by Verticillium dahliae inoculation and was responsive to defense signaling molecules, including ethylene, jas- monic acid, and salicylic acid. Knockdown of GhMLP28 expression by virus-induced gene silencing re- sulted in increased susceptibility of cotton plants to V. dahliae infection, while ectopic overexpression of GhMLP28 in tobacco improved the disease tolerance of the transgenic plants. Further analysis revealed that GhMLP28 interacted with cotton ethylene response factor 6 （GhERF6） and facilitated the binding of GhERF6 to GCC-box element. Transient expression assay demonstrated that GhMLP28 enhanced the tran- scription factor activity of GhERF6, which led to the augmented expression of some GCC-box genes. GhMLP28 proteins were located in both the nucleus and cytoplasm and their nuclear distribution was dependent on the presence of GhERF6. Collectively, these results demonstrate that GhMLP28 acts as a positive regulator of GhERF6, and synergetic actions of the two proteins may contribute substantially to protection against V. dahliae infection in cotton plants.