Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation

Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correla...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1994-10, Vol.91 (22), p.10502-10506
Main Authors: Ingelbrecht, I. (Universiteit Gent, Ghent, Belgium), Houdt, H. van, Montagu, M. van, Depicker, A
Format: Article
Language:English
Subjects:
ADN
Arabidopsis - genetics
Arabidopsis - metabolism
Base Sequence
Biochemistry
Caulimovirus
Cell Nucleus - metabolism
CITOSINA
CYTOSINE
DNA
DNA Primers
DNA, Bacterial - metabolism
DNA, Ribosomal - metabolism
EXPRESION GENICA
EXPRESSION DES GENES
FOSFOTRASFERASA
GENE
Gene expression
Gene Expression Regulation
Gene silencing
GENES
Genetic loci
Genetic Vectors
GENETICA
GENETIQUE
Methylation
Molecular Sequence Data
Nicotiana - genetics
Nicotiana - metabolism
NICOTIANA TABACUM
PHOSPHOTRANSFERASE
Plant cells
Plant Leaves
Plants, Genetically Modified
Plants, Toxic
Plasmids
Polymerase Chain Reaction
Rhizobium
RNA
RNA Processing, Post-Transcriptional
Sequence Homology, Nucleic Acid
Tobacco
Transcription, Genetic
Transgenes
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Description
Summary:Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical motifs CG and CXG
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.91.22.10502