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Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation
Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correla...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 1994-10, Vol.91 (22), p.10502-10506 |
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| Main Authors: | , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c544t-242c7f8016bf089f96150e518f67965b43cbdff611fcb43da431c1cab38bac7c3 |
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| container_end_page | 10506 |
| container_issue | 22 |
| container_start_page | 10502 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 91 |
| creator | Ingelbrecht, I. (Universiteit Gent, Ghent, Belgium) Houdt, H. van Montagu, M. van Depicker, A |
| description | Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical motifs CG and CXG |
| doi_str_mv | 10.1073/pnas.91.22.10502 |
| format | article |
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Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical motifs CG and CXG</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.91.22.10502</identifier><identifier>PMID: 7937983</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>ADN ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Base Sequence ; Biochemistry ; Caulimovirus ; Cell Nucleus - metabolism ; CITOSINA ; CYTOSINE ; DNA ; DNA Primers ; DNA, Bacterial - metabolism ; DNA, Ribosomal - metabolism ; EXPRESION GENICA ; EXPRESSION DES GENES ; FOSFOTRASFERASA ; GENE ; Gene expression ; Gene Expression Regulation ; Gene silencing ; GENES ; Genetic loci ; Genetic Vectors ; GENETICA ; GENETIQUE ; Methylation ; Molecular Sequence Data ; Nicotiana - genetics ; Nicotiana - metabolism ; NICOTIANA TABACUM ; PHOSPHOTRANSFERASE ; Plant cells ; Plant Leaves ; Plants, Genetically Modified ; Plants, Toxic ; Plasmids ; Polymerase Chain Reaction ; Rhizobium ; RNA ; RNA Processing, Post-Transcriptional ; Sequence Homology, Nucleic Acid ; Tobacco ; Transcription, Genetic ; Transgenes</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1994-10, Vol.91 (22), p.10502-10506</ispartof><rights>Copyright 1994 The National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Oct 25, 1994</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c544t-242c7f8016bf089f96150e518f67965b43cbdff611fcb43da431c1cab38bac7c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/91/22.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2366057$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2366057$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793,58238,58471</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7937983$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ingelbrecht, I. (Universiteit Gent, Ghent, Belgium)</creatorcontrib><creatorcontrib>Houdt, H. van</creatorcontrib><creatorcontrib>Montagu, M. van</creatorcontrib><creatorcontrib>Depicker, A</creatorcontrib><title>Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical motifs CG and CXG</description><subject>ADN</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>Caulimovirus</subject><subject>Cell Nucleus - metabolism</subject><subject>CITOSINA</subject><subject>CYTOSINE</subject><subject>DNA</subject><subject>DNA Primers</subject><subject>DNA, Bacterial - metabolism</subject><subject>DNA, Ribosomal - metabolism</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>FOSFOTRASFERASA</subject><subject>GENE</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Gene silencing</subject><subject>GENES</subject><subject>Genetic loci</subject><subject>Genetic Vectors</subject><subject>GENETICA</subject><subject>GENETIQUE</subject><subject>Methylation</subject><subject>Molecular Sequence Data</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>NICOTIANA TABACUM</subject><subject>PHOSPHOTRANSFERASE</subject><subject>Plant cells</subject><subject>Plant Leaves</subject><subject>Plants, Genetically Modified</subject><subject>Plants, Toxic</subject><subject>Plasmids</subject><subject>Polymerase Chain Reaction</subject><subject>Rhizobium</subject><subject>RNA</subject><subject>RNA Processing, Post-Transcriptional</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Tobacco</subject><subject>Transcription, Genetic</subject><subject>Transgenes</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNp9kc9rFDEcxYModa3eRRQHD9LLrPmdCXgpVVuhqKA9h0w22c0ym0yTjNr_vtnuulgPnsL3-z7vyyMPgOcIzhEU5N0YdJ5LNMe4zgziB2CGoEQtpxI-BDMIsWg7iulj8CTnNYRQsg4egSMhiZAdmQH1LeZSkg7ZJD8WH4MemuwHG4wPyya6JtkxpmJTc0ctbbC58aEpsdfGxMbElOygS93-8mXVfPhy2mxsWd3UXb32FDxyesj22f49BlefPv44u2gvv55_Pju9bA2jtLSYYiNcBxHvHeykkxwxaBnqHBeSs54S0y-c4wg5U4eFpgQZZHRPuhpDGHIM3u_ujlO_sQtjQ407qDH5jU43Kmqv7ivBr9Qy_lSUQSqr_e3enuL1ZHNRG5-NHQYdbJyyQpwjKjiv4Jt_wHWcUv20rDBEWHBCcYXgDjIp5pysO-RAUG17U9velEQKY3XXW7W8-jv_wbAvquqv9_rW-Ue9f-Hk_4Ry0zAU-7tU9OUOXecS04HFhHPIRJVf7GSno9LL5LO6-i4ZFqzj5BYGkMDm</recordid><startdate>19941025</startdate><enddate>19941025</enddate><creator>Ingelbrecht, I. 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(Universiteit Gent, Ghent, Belgium)</au><au>Houdt, H. van</au><au>Montagu, M. van</au><au>Depicker, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1994-10-25</date><risdate>1994</risdate><volume>91</volume><issue>22</issue><spage>10502</spage><epage>10506</epage><pages>10502-10506</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical motifs CG and CXG</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>7937983</pmid><doi>10.1073/pnas.91.22.10502</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1994-10, Vol.91 (22), p.10502-10506 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16614766 |
| source | PubMed Central(OpenAccess); JSTOR Archival Journals and Primary Sources Collection |
| subjects | ADN Arabidopsis - genetics Arabidopsis - metabolism Base Sequence Biochemistry Caulimovirus Cell Nucleus - metabolism CITOSINA CYTOSINE DNA DNA Primers DNA, Bacterial - metabolism DNA, Ribosomal - metabolism EXPRESION GENICA EXPRESSION DES GENES FOSFOTRASFERASA GENE Gene expression Gene Expression Regulation Gene silencing GENES Genetic loci Genetic Vectors GENETICA GENETIQUE Methylation Molecular Sequence Data Nicotiana - genetics Nicotiana - metabolism NICOTIANA TABACUM PHOSPHOTRANSFERASE Plant cells Plant Leaves Plants, Genetically Modified Plants, Toxic Plasmids Polymerase Chain Reaction Rhizobium RNA RNA Processing, Post-Transcriptional Sequence Homology, Nucleic Acid Tobacco Transcription, Genetic Transgenes |
| title | Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation |
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