Immobilisation of bromoperoxidase from Corallina officinalis

The vanadium-dependent bromoperoxidase from the macroalga Corallina officinalis was immobilised on a cellulose acetate support with retention of approaching 50% of the applied units of activity. The enzyme exhibited high thermal stability and retained activity in repeated use. The immobilised enzyme...

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Bibliographic Details
Published in:Biotechnology techniques 1994-08, Vol.8 (8), p.579-582
Main Authors: Sheffield, D.J, Harry, T.R, Smith, A.J, Rogers, L.J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
cellulose acetate
Corallina officinalis
enzyme activity
Fundamental and applied biological sciences. Psychology
immobilization
Immobilization of enzymes and other molecules
Immobilization techniques
immobilized enzymes
Methods. Procedures. Technologies
peroxidases
Rhodophycota
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Summary:The vanadium-dependent bromoperoxidase from the macroalga Corallina officinalis was immobilised on a cellulose acetate support with retention of approaching 50% of the applied units of activity. The enzyme exhibited high thermal stability and retained activity in repeated use. The immobilised enzyme showed tolerance to organic solvents similar to that of the free enzyme in the case of methanol but differed for acetone and ethanol, and with the latter showed enhanced activity as the % by volume of the solvent was increased.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00152150