Simultaneous detection of dehydroascorbic, ascorbic, and some organic acids in fruits and vegetables by HPLC

A high-performance liquid chromatography method was developed for the simultaneous determination of dehydroascorbic, ascorbic, malic, citric, and oxalic acids in fruits, vegetables, and beverages. Separation of these compounds was accomplished by coupling reversed-phase and organic acid columns usin...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 1992-07, Vol.40 (7), p.1127-1130
Main Authors: Nisperos-Carriedo, Myrna O, Buslig, Bela S, Shaw, Philip E
Format: Article
Language:English
Subjects:
acide ascorbique
acide organique
acido ascorbico
acidos organicos
agrume
ascorbic acid
averrhoa carambola
Biological and medical sciences
capsicum
carica papaya
chemical composition
chromatographie liquide haute press
citrus fruits
composicion quimica
composition chimique
cromatografia liquida alta presion
Food industries
Fruit and vegetable industries
frutas citricas
Fundamental and applied biological sciences. Psychology
high pressure liquid chromatography
mango
mangoes
mangue
organic acids
tomate
tomatoes
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A high-performance liquid chromatography method was developed for the simultaneous determination of dehydroascorbic, ascorbic, malic, citric, and oxalic acids in fruits, vegetables, and beverages. Separation of these compounds was accomplished by coupling reversed-phase and organic acid columns using 2% KH2PO4 (pH 2.3) as mobile phase with a flow rate of 0.4 mL/min. Detection was performed at 215 and 260 nm using a diode array detector interfaced with portable integrators and a chromatography data system. Selected fresh fruits, vegetables, and commercial orange juices were analyzed using this method.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00019a007