Restriction fragment length polymorphism differences among Illinois long-term selection oil strains

• Published in: Theoretical and applied genetics 1994-03, Vol.87 (8), p.916-924
• Main Authors: Sughroue, J.R, Rocheford, T.R. (Illinois Univ., Urbana (USA). Dept. of Agronomy)
• Format: Article
• Language: English
• Subjects: ADN; Agronomy. Soil science and plant productions; Biological and medical sciences; Classical genetics, quantitative genetics, hybrids; CONTENIDO DE LIPIDOS; ENZIMA DE RESTRICCION; ENZYME DE RESTRICTION; EPREUVE SUR LA DESCENDANCE; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; LIPIDE; LIPIDOS; POLIMORFISMO GENETICO; POLYMORPHISME GENETIQUE; PRUEBAS DE DESCENDENCIA; Pteridophyta, spermatophyta; SECUENCIA NUCLEOTIDICA; SELECCION; SELECTION; SEQUENCE NUCLEOTIDIQUE; TENEUR EN LIPIDES; Vegetals; ZEA MAYS
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/BF00225785

Restriction fragment length polymorphism (RFLP) analysis was used to characterize variability in the Illinois Long-Term Selection Experiment oil strains. Considerable polymorphism was detected within each oil strain and among oil strains. 52 individual plants from each of the Illinois High Oil (IHO), Illinois Low Oil (ILO), Reverse High Oil (RHO) and Reverse Low Oil (RLO) strains were sampled to determine RFLP allele/variant frequencies. Generation 90 was sampled for IHO, RHO, and RLO whereas generation 87 was sampled for ILO. 49 RFLP probes distributed throughout the maize genome were used. Chi-square analysis was performed to determine if RFLP genotypes at each of the 49 RFLP loci were significantly different among strains. Oil strains that have been separated for 90 generations showed high levels of significantly-different RFLP genotypic frequencies. The comparison of ILO vs RHO gave only significant chi-square values while the comparisons of IHO vs RLO and RHO vs RLO had 11:1 ratios of significant to non-significant chi-square values. Strains that have been separated for only 42 generations showed a lower level of significantly-different RFLP genotypic frequencies. The comparisons of IHO vs RHO and ILO vs RLO both had only a 3:2 ratio of significant to non-significant chi-squares values. Detection of multiple RFLP alleles/variants among the oil strains was common with 59% of the RFLP loci examined exhibiting multiple variants. A number of RFLP loci in RHO (3) and RLO (11) were associated with a trend in RFLP allele/variant frequencies consistent with a response to reverse selection for oil concentration.