A longitudinal proteomic assessment of peptide degradation and loss under acidic storage conditions

Sample preparation prior to analysis by liquid chromatography electrospray ionization mass spectrometry (LC–ESI–MS) usually involves the storage of frozen peptide samples in an acidic environment for variable time periods. Questions arose in our laboratory regarding the stability of peptides in acid...

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Bibliographic Details
Published in:Analytical biochemistry 2015-03, Vol.473, p.11-13
Main Authors: Planyavsky, Melanie, Huber, Marie L., Staller, Nico A., Müller, André C., Bennett, Keiryn L.
Format: Article
Language:English
Subjects:
Acid degradation
Amino Acid Sequence
Analytic Sample Preparation Methods
Animals
Cattle
Cold Temperature
Drug Stability
Hydrogen-Ion Concentration
Orbitrap Velos mass spectrometer
Peptide stability
Peptide storage
Peptides - chemistry
Proteolysis
Proteomics
Storage temperature
Time Factors
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Summary:Sample preparation prior to analysis by liquid chromatography electrospray ionization mass spectrometry (LC–ESI–MS) usually involves the storage of frozen peptide samples in an acidic environment for variable time periods. Questions arose in our laboratory regarding the stability of peptides in acid under medium- to long-term storage. Thus, a 10-month longitudinal study was designed to assess the effect on storage of tryptic peptides at −20 and −80°C under acidic conditions. Our conclusion and proposal from this evaluation is that the optimal storage conditions of peptide samples in acid for proteomic experiments is at −80°C and, ideally, as separate aliquots.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2014.11.020