High Concentration of Insulin Promotes Apoptosis of Primary Cultured Rat Ovarian Granulosa Cells Via Its Increase in Extracellular HMGB1

Polycystic ovary syndrome (PCOS) is a common endocrine disorder affecting women of reproductive age. Insulin resistance/hyperinsulinemia is a prevalent finding in women with PCOS, which indicates that insulin resistance/hyperinsulinemia may be an important player in the pathogenesis of the PCOS. How...

Full description

Saved in:
Bibliographic Details
Published in:Reproductive sciences (Thousand Oaks, Calif.) Calif.), 2015-03, Vol.22 (3), p.271-277
Main Authors: Ni, Xiao-Rong, Sun, Zhou-Jun, Hu, Guo-Hua, Wang, Rong-Hui
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Case-Control Studies
Cells, Cultured
Dose-Response Relationship, Drug
Embryology
Female
Granulosa Cells - drug effects
Granulosa Cells - metabolism
Granulosa Cells - pathology
HMGB1 Protein - blood
HMGB1 Protein - genetics
HMGB1 Protein - metabolism
Humans
Insulin - toxicity
Insulin Resistance
Medicine & Public Health
Obstetrics/Perinatology/Midwifery
Polycystic Ovary Syndrome - blood
Polycystic Ovary Syndrome - physiopathology
Rats, Sprague-Dawley
Reproductive Medicine
Review
RNA Interference
Signal Transduction - drug effects
Time Factors
Transfection
Up-Regulation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Polycystic ovary syndrome (PCOS) is a common endocrine disorder affecting women of reproductive age. Insulin resistance/hyperinsulinemia is a prevalent finding in women with PCOS, which indicates that insulin resistance/hyperinsulinemia may be an important player in the pathogenesis of the PCOS. However, the underlying mechanism of insulin resistance/hyperinsulinemia on the pathogenesis of the PCOS remains elusive. In this study, we found an increased high-mobility group box 1 (HMGB1) in the serum from women with PCOS having insulin resistance/hyperinsulinemia. Furthermore, we discovered that high concentration of insulin, which mimics insulin resistance model, promoted apoptosis in primary cultured rat ovarian granulosa cells (GCs) via its effect on the increase in extracellular HMGB1. Our data presented the first evidence that increased HMGB1 induced by insulin resistance/hyperinsulinemia promoted apoptosis of ovarian GCs, which provided new molecular basis for the PCOS pathogenesis.
ISSN:1933-7191
1933-7205
DOI:10.1177/1933719114549852