Shenfu injection protects human ECV304 cells from hydrogen peroxide via its anti-apoptosis way

• Published in: Journal of ethnopharmacology 2015-04, Vol.163, p.203-209
• Main Authors: Fen-fang, Hong, Fa-xian, Guo, Ying, Zhou, Qin-hua, Min, Da-lei, Zhang, Bei, Yang, Wei-ying, Zhou, Lei, Wu, Zhi-ping, Wei, Hui, Liu, Shu-long, Yang
• Format: Article
• Language: English
• Subjects: Anti-oxidation; Antioxidants - pharmacology; Apoptosis; Apoptosis - drug effects; bcl-2-Associated X Protein - metabolism; Caspase 3 - metabolism; Cell Line; Drugs, Chinese Herbal; H2O2; Humans; Hydrogen Peroxide; Injections; Oxidative Stress - drug effects; Proto-Oncogene Proteins c-bcl-2 - metabolism; Shenfu injection; Vascular endothelial cell
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2015.01.032

The pathogenesis of thromboangiitis obliterans (TAO) has not been fully elucidated until now. Shenfu injection (SFI), a traditional Chinese formula has been widely used clinically for the treatment of cardiovascular diseases for more than two decade. Our previous results first suggested that SFI can cause a significant therapeutic effect on experimental TAO model rats. This experiment was designed to further investigate the protective effect of SFI on VEC damaged by hydrogen peroxide (H2O2) oxidative stress in vitro. The cell viability was evaluated by the MTT assay, the activities of SOD and GSH-PX and the content of MDA in the supernatants of the cultured ECV304 cells were evaluated by a colorimetry method, cell apoptosis was detected by flow cytometry and an AO/EB double staining method. The protein expressions of Bcl2, Bax and caspase-3 were examined by Western blotting. When compared with control group, lower survival rate of ECV304 cells was observed in H2O2 group (p