Temporal variation of faecal indicator bacteria in tropical urban storm drains

• Published in: Water research (Oxford) 2015-01, Vol.68, p.171-181
• Main Authors: Ekklesia, E., Shanahan, P., Chua, L.H.C., Eikaas, H.S.
• Format: Article
• Language: English
• Subjects: Bacteria; Bacteria - isolation & purification; Contamination; Diurnal variation; Environmental Monitoring; Faecal indicator bacteria; Feces - microbiology; Indicators; Sampling; Sewage - analysis; Sewers; Singapore; Surface water; Time Factors; Tropical Climate; Urban areas; Urban storm drain; Waste Water - microbiology; Water quality
• ISSN: 0043-1354; 1879-2448
• DOI: 10.1016/j.watres.2014.09.049

Human faecal contamination poses a widespread hazard for human health. In urban areas, sewer leakage may be an important cause of faecal pollution to surface water. Faecal indicator bacteria (FIB) are the most widely used indicators to monitor surface water quality. However, assessing whether a water body is meeting water quality criteria is made difficult by the high variability of FIB concentrations over time. In this study, the variation of FIB concentration in surface water from tropical urban catchments is investigated. Eleven urban sub-catchments were sampled hourly over 24-hr and samples analysed for FIB. It was found that FIB show a diurnal pattern that is characterised by daytime FIB concentrations that are significantly higher than nighttime FIB concentrations. This observed diurnal variation of FIB closely follows that of sewer flows and contrasts with observations in rural streams where FIB concentrations are known to be low in the daytime and high during the night. Field tracer tests provide qualitative evidence of sewage exfiltration and transport to drains via preferential flow paths. The diurnal FIB variation and field tracer tests indicate the likelihood of surface water contamination due to leaking sewers. The results further suggest that contamination of surface-water drains is likely a widespread problem in tropical urban areas due to extensive drainage networks and the persistence of FIB under tropical conditions. Because of FIB variation over time, the time at which samples are collected is important in being able to capture the daily maximum and minimum FIB concentrations. The Kruskal–Wallis test shows that hourly sampling from 04:00 to 07:00 and from 12:00 to 15:00 results in significantly different FIB concentration (minimum and maximum, respectively). Furthermore, the Wilcoxon-Mann-Whitney test shows that sampling at 12:00 and 14:00 results in significantly higher FIB concentrations, while sampling at 05:00 and 04:00 or 05:00 and 06:00 results in significantly lower FIB concentrations, than sampling at other hours of the day. [Display omitted] •Dry-weather hourly sampling over 24 h was conducted at urban storm drains.•FIB concentrations were high during daytime and low during nighttime.•The observed FIB diurnal variation closely follows that of sewer flows.•Sampling times are important to capture the variation.