A new selection method for isolating mutants defective in acetate utilisation in Aspergillus nidulans

Propionate medium is normally toxic for the growth of Aspergillus nidulans. Spontaneous mutations relieving the toxicity to propionate, which arose on propionate medium, have been shown to be mutations in acetate metabolism. One acu- mutant is allelic with acuA (the structural gene for acetyl-CoA sy...

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Bibliographic Details
Published in:Current genetics 1994, Vol.25 (1), p.47-48
Main Author: Sealy-Lewis, H.M. (Hull Univ. (United Kingdom). Dept. of Applicd Biology)
Format: Article
Language:English
Subjects:
ACETATE
Acetates - metabolism
ACETATOS
Acetic Acid
ANALISIS MICROBIOLOGICO
ANALYSE MICROBIOLOGIQUE
ASPERGILLUS NIDULANS
Aspergillus nidulans - drug effects
Aspergillus nidulans - genetics
Aspergillus nidulans - metabolism
Biological Transport, Active - genetics
Culture Media
Genes, Fungal
Genetic Techniques
METABOLISME
METABOLISMO
Mutagenesis
Mutation
PROPIONATE
Propionate metabolism
Propionates - metabolism
Propionates - toxicity
PROPIONATOS
SELECCION
SELECTION
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Summary:Propionate medium is normally toxic for the growth of Aspergillus nidulans. Spontaneous mutations relieving the toxicity to propionate, which arose on propionate medium, have been shown to be mutations in acetate metabolism. One acu- mutant is allelic with acuA (the structural gene for acetyl-CoA synthetase), another with acuB (the regulatory gene involved in the induction of enzymes concerned with acetate metabolism, including acetyl-CoA synthetase), and a third mutant, acuO, represents a new acu- locus that maps on linkage group V.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00712966