Anantin - A peptide antagonist of the atrial natriuretic factor (ANF). 1. Producing organism, fermentation, isolation and biological activity

Anantin, a peptide binding to the receptor of the atrial natriuretic factor (ANF) was isolated from a strain of Streptomyces coerulescens . The molecule consists of 17 natural L-amino acids which form a peptidic ring system. It has a MW of 1,871.0. The chemical composition is C sub(90)H sub(111)-N s...

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Bibliographic Details
Published in:Journal of antibiotics 1991-02, Vol.44 (2), p.164-171
Main Authors: Weber, W, Fischli, W, Hochuli, E, Kupfer, E, Weibel, E K
Format: Article
Language:English
Online Access:Get full text
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Summary:Anantin, a peptide binding to the receptor of the atrial natriuretic factor (ANF) was isolated from a strain of Streptomyces coerulescens . The molecule consists of 17 natural L-amino acids which form a peptidic ring system. It has a MW of 1,871.0. The chemical composition is C sub(90)H sub(111)-N sub(21)O sub(24). The compound was found to bind competitively to ANF-receptors from bovine adrenal cortex (K sub(d) = 0.61 mu M). Furthermore, it dose-dependently inhibited the ANF-induced intracellular cyclic guanosine monophosphate accumulation in bovine aorta smooth muscle cells. At the same concentration no agonistic effects were detectable in these cells. Thus, anantin is considered to be the first microbially produced antagonist of the cardiac hormone, ANF.
ISSN:0021-8820
DOI:10.7164/antibiotics.44.164