In vivo quantitative magnetization transfer imaging correlates with histology during de- and remyelination in cuprizone-treated mice

The pool size ratio measured by quantitative magnetization transfer MRI is hypothesized to closely reflect myelin density, but their relationship has so far been confirmed mostly in ex vivo conditions. We investigate the correspondence between this parameter measured in vivo at 7.0 T, with Black Gol...

Full description

Saved in:
Bibliographic Details
Published in:NMR in biomedicine 2015-03, Vol.28 (3), p.327-337
Main Authors: Turati, Laura, Moscatelli, Marco, Mastropietro, Alfonso, Dowell, Nicholas G., Zucca, Ileana, Erbetta, Alessandra, Cordiglieri, Chiara, Brenna, Greta, Bianchi, Beatrice, Mantegazza, Renato, Cercignani, Mara, Baggi, Fulvio, Minati, Ludovico
Format: Article
Language:English
Subjects:
animal model
Animals
Brain - metabolism
Brain - pathology
Cuprizone
demyelination
Female
macromolecular pool size ratio (F)
Magnetic Phenomena
Magnetic Resonance Imaging
Male
Mice, Inbred C57BL
multiple sclerosis
Myelin Sheath - metabolism
Myelin Sheath - pathology
quantitative magnetization transfer imaging (qMT)
remyelination
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The pool size ratio measured by quantitative magnetization transfer MRI is hypothesized to closely reflect myelin density, but their relationship has so far been confirmed mostly in ex vivo conditions. We investigate the correspondence between this parameter measured in vivo at 7.0 T, with Black Gold II staining for myelin fibres, and with myelin basic protein and beta‐tubulin immunofluorescence in a hybrid longitudinal study of C57BL/6 and SJL/J mice treated with cuprizone, a neurotoxicant causing relatively selective myelin loss followed by spontaneous remyelination upon treatment suspension. Our results confirm that pool size ratio measurements correlate with myelin content, with the correlation coefficient depending on strain and staining method, and demonstrate the in vivo applicability of this MRI technique to experimental mouse models of multiple sclerosis. Copyright © 2015 John Wiley & Sons, Ltd. The relationship between the pool size ratio measured by quantitative magnetization‐transfer MRI and myelin histology has so far been confirmed mostly in ex vivo conditions. We investigated the correspondence between these two parameters at 7.0 T on C57BL/6 and SJL/J mice treated with cuprizone, a neurotoxicant causing primarily myelin loss. We confirm that pool size ratio measurements correlate with myelin content.
ISSN:0952-3480
1099-1492
DOI:10.1002/nbm.3253