The role of endoglucanase and endoxylanase in liquefaction of hydrothermally pretreated wheat straw

The role of endocellulases and endoxylanase during liquefaction and saccharification of hydrothermally pretreated wheat straw was studied. The use of a flow‐loop setup with in‐line magnetic resonance imaging enabled frequent measurements of viscosity at 55°C during saccharification at 6% total solid...

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Bibliographic Details
Published in:Biotechnology progress 2014-07, Vol.30 (4), p.923-931
Main Authors: Skovgaard, Pernille Anastasia, Thygesen, Lisbeth Garbrecht, Jørgensen, Henning, Cardona, Maria, Tozzi, Emilio, McCarthy, Michael, Siika-Aho, Matti, Jeoh, Tina
Format: Article
Language:English
Subjects:
Carbohydrates - chemistry
Cellulase - chemistry
Cellulases - chemistry
Endo-1,4-beta Xylanases - chemistry
endoglucanase
endoxylanase
Fermentation
fiber attrition
Hydrolysis
Magnetic Resonance Imaging
thermostable enzymes
Triticum - chemistry
Triticum aestivum
Viscosity
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Summary:The role of endocellulases and endoxylanase during liquefaction and saccharification of hydrothermally pretreated wheat straw was studied. The use of a flow‐loop setup with in‐line magnetic resonance imaging enabled frequent measurements of viscosity at 55°C during saccharification at 6% total solids content. Viscosity data were complemented with off‐line measurements of fiber lengths and release of soluble sugars. A clear correlation between fiber attrition and a decrease in viscosity was found. Fiber lengths and viscosity dropped quickly within the first hour and then stagnated, while sugar yields increased substantially thereafter, illustrating that liquefaction and saccharification are separate mechanisms. Both endoglucanase and endoxylanase were shown to have a significant effect on viscosity during liquefaction while the addition of endoxylanase also increased sugar yield. © 2014 American Institute of Chemical Engineers Biotechnol. Prog., 30:923–931, 2014
ISSN:8756-7938
1520-6033
DOI:10.1002/btpr.1893