Immobilization of enzyme to platinum electrode and its use as enzyme electrode

A glucose electrode was fabricated by immobilizing glucose oxidase covalently onto a platinized platinum electrode. The sensor showed rapid response with response time of 2-4 s, and also the linear response to the glucose concentration, ranging from 2 x 10 super(-3) to 5 mM. The sensitivity was foun...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1991-03, Vol.28 (1), p.211-219
Main Authors: Kawakami, M. (Fukuoka Institute of Technology, Fukuoka, Japan), Koya, H, Gondo, S
Format: Article
Language:English
Subjects:
BIODETECTEUR
BIODETECTOR
Biological and medical sciences
BIOSENSORS
Biotechnology
ELECTRODE
ELECTRODES
ELECTRODOS
Fundamental and applied biological sciences. Psychology
GALACTOSA
GALACTOSE
GLUCOSA OXIDASA
GLUCOSE OXIDASE
GLUCOSE OXYDASE
IMMOBILISATION
IMMOBILIZATION
Immobilization of enzymes and other molecules
Immobilization techniques
INMOVILIZACION
Methods. Procedures. Technologies
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Summary:A glucose electrode was fabricated by immobilizing glucose oxidase covalently onto a platinized platinum electrode. The sensor showed rapid response with response time of 2-4 s, and also the linear response to the glucose concentration, ranging from 2 x 10 super(-3) to 5 mM. The sensitivity was found to be correlated with the surface area of a base electrode used. (DBO)
ISSN:0273-2289
1559-0291
DOI:10.1007/BF02922602